Multi-dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells.
Multi-dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells.
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DOI：
10.1007/978-1-0716-2269-8_2
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发表时间：
2022
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有助于我们理解细胞生物学的荧光活细胞成像现在处于研究细胞定量生物化学的前沿。特别是，近年来荧光活细胞成像的技术进步和相关策略使我们能够在活的人类细胞中发现各种亚细胞大分子组装。在这里，我们描述了如何在单个人类细胞的2维和3维空间中研究负责在亚细胞水平调节葡萄糖流量的多酶代谢组件--“葡萄糖体”的实时动力学。我们预计，这种多维荧光活细胞成像将继续彻底改变我们对细胞内代谢途径及其网络是如何在单细胞水平上进行功能协调的理解。
Fluorescence live-cell imaging that has contributed to our understanding of cell biology is now at the frontline of studying quantitative biochemistry in a cell. Particularly, the technological advancement of fluorescence live-cell imaging and associated strategies in recent years have allowed us to discover various subcellular macromolecular assemblies in living human cells. Here we describe how the real-time dynamics of a multienzyme metabolic assembly, the “glucosome,” that is responsible for regulating glucose flux at subcellular levels, has been investigated in both 2- and 3-dimensional space of single human cells. We envision that such multi-dimensional fluorescence live-cell imaging will continue to revolutionize our understanding of how intracellular metabolic pathways and their network are functionally orchestrated at single-cell levels.