Uncoordinated (UNC)119: coordinating the trafficking of myristoylated proteins.
Uncoordinated (UNC)119: coordinating the trafficking of myristoylated proteins.
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DOI：
10.1016/j.visres.2012.08.012
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发表时间：
2012-12-15
期刊：
影响因子：
1.8
通讯作者：
Baehr, Wolfgang
中科院分区：
文献类型：
--
作者：
Constantine, Ryan;Zhang, Houbin;Gerstner, Cecilia D.;Frederick, Jeanne M.;Baehr, Wolfgang
关键词：
肉豆蔻酰化蛋白靶向特定亚细胞膜区室的机制尚不清楚。最近显示，两种新型酰基结合蛋白 UNC119A 和 UNC119B 在肉豆蔻酰化 G 蛋白 α 亚基和 src 型酪氨酸激酶的转运中充当伴侣/辅助因子。 UNC119 多肽具有类似免疫球蛋白的 β-夹心折叠，形成能够结合月桂酰 (C12) 和肉豆蔻酰 (C14) 侧链的疏水口袋。视杆光感受器中的 UNC119A 通过形成中间可扩散的 UNC119-Tα 复合物，促进转导蛋白 α 亚基 (Tα) 从内节膜转移到外节膜。由于线虫 unc-119 突变体中的 G 蛋白 ODR-3 和 GPA-13 运输不当，其他感觉神经元中也形成了类似的复合物。 IMCD3 细胞中的 UNC119B 敲低可阻止肉豆蔻酰化肾囊肿蛋白 3 (NPHP3)（一种与肾结核相关的蛋白质）转运至纤毛。此外，UNC119A 可将肉豆蔻酰化 src 型酪氨酸激酶转运至细胞膜并影响 T 细胞受体 (TCR) 和白细胞介素 5 受体 (IL-5R) 活性。这些相互作用使 UNC119 多肽成为新型脂质结合伴侣，对肉豆蔻酰化蛋白的不同子集具有特异性。
The mechanism by which myristoylated proteins are targeted to specific subcellular membrane compartments is poorly understood. Two novel acyl-binding proteins, UNC119A and UNC119B, have been shown recently to function as chaperones/co-factors in the transport of myristoylated G protein α-subunits and src-type tyrosine kinases. UNC119 polypeptides feature an immunoglobulin-like β-sandwich fold that forms a hydrophobic pocket capable of binding lauroyl (C12) and myristoyl (C14) side chains. UNC119A in rod photoreceptors facilitates the transfer of transducin α subunits (Tα) from inner segment to outer segment membranes by forming an intermediate diffusible UNC119-Tα complex. Similar complexes are formed in other sensory neurons, as the G proteins ODR-3 and GPA-13 in C. elegans unc-119 mutants traffic inappropriately. UNC119B knockdown in IMCD3 cells prevents trafficking of myristoylated nephrocystin-3 (NPHP3), a protein associated with nephronophthisis, to cilia. Further, UNC119A was shown to transpot myristoylated src-type tyrosine kinases to cell membranes and to affect T-cell receptor (TCR) and interleukin-5 receptor (IL-5R) activities. These interactions establish UNC119 polypeptides as novel lipid-binding chaperones with specificity for a diverse subset of myristoylated proteins.
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DOI：
10.1084/jem.20030589
发表时间：
2004-02-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gorska MM;Stafford SJ;Cen O;Sur S;Alam R
通讯作者：
Alam R
影响因子：
14.8
作者：
Ismail, Shehab A.;Chen, Yong-Xiang;Wittinghofer, Alfred
通讯作者：
Wittinghofer, Alfred
影响因子：
3.4
作者：
Ishiba, Yasutsugu;Higashide, Tornorni;Inana, George
通讯作者：
Inana, George
影响因子：
3.3
作者：
Keller, Lani C.;Geimer, Stefan;Marshall, Wallace F.
通讯作者：
Marshall, Wallace F.
影响因子：
4.8
作者：
Cen, O;Gorska, MM;Alam, R
通讯作者：
Alam, R