CHROMOSOMAL LOCATION AND CLONING OF THE GENE (TRMD) RESPONSIBLE FOR THE SYNTHESIS OF TRANSFER RNA(M1G)METHYLTRANSFERASE IN ESCHERICHIA-COLI K-12
CHROMOSOMAL LOCATION AND CLONING OF THE GENE (TRMD) RESPONSIBLE FOR THE SYNTHESIS OF TRANSFER RNA(M1G)METHYLTRANSFERASE IN ESCHERICHIA-COLI K-12
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DOI：
10.1007/bf00330046
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
BJORK, GR
中科院分区：
文献类型：
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作者：
BYSTROM, AS;BJORK, GR
控制tRNA中1-甲基鸟苷(M1G)形成的trmD基因是通过噬菌体P1转导定位在大肠杆菌染色体图谱上的。56min时共转导到Tyra的比例为80%。从Clarke和Carbon的收集中分离到一个ColE1-Tyra+杂交质粒，它携带trmD+基因，并被证明能过量生产tRNA(M1G)甲基转移酶。通过体外亚克隆限制性内切酶片段，将trmD+基因定位于Tyra+基因顺时针方向的3.4kb[kbase]DNA片段6.5kb。突变trmD1使tRNA(M1G)甲基转移酶在体内和体外对温度敏感，可由trmD+质粒补充。这些结果表明，trmD+基因是tRNA(M1G)甲基转移酶(EC2.1.1.3.1)的结构基因。
The trmD gene, which governs the formation of 1-methyl-guanosine(m1G) in tRNA, was located by phage P1 transduction at 56 min on the chromosomal map of E. coli. Cotransduction to tyrA at 56 min is 80%. From the Clarke and Carbon collection, a ColE1-tyrA+ hybrid plasmid was isolated, which carried the trmD+ gene and was shown to overproduce the tRNA(m1G)methyltransferase. By subcloning restriction enzyme fragments in vitro, the trmD+ gene was located to a 3.4 kb [kilobase] DNA fragment 6.5 kb clockwise from the tyrA+ gene. The mutation trmD1, which renders the tRNA(m1G)methyltransferase temperature-sensitive in vivo and in vitro could be complemented by trmD+ plasmids. These results suggest that the gene trmD+ is the structural gene for the tRNA (m1G)methyltransferase (EC 2.1.1.3.1).