Exposure to atrazine during puberty reduces sperm viability, increases weight gain and alters the expression of key metabolic genes in the liver of male mice
Exposure to atrazine during puberty reduces sperm viability, increases weight gain and alters the expression of key metabolic genes in the liver of male mice
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DOI：
10.1071/rd18505
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发表时间：
2019-04-01
影响因子：
1.9
通讯作者：
Pask, Andrew J.
中科院分区：
文献类型：
--
作者：
Cook, Laura E.;Finger, Bethany J.;Pask, Andrew J.
阿特拉津(ATZ)是世界上使用最广泛的除草剂之一，也是人类饮用水中常见的污染物。它扰乱了植物的代谢途径，并对包括人类在内的脊椎动物的代谢和生殖产生影响。很少有研究调查暴露于低剂量ATZ的影响，特别是在男性的性发育期间。在这项研究中，我们将断奶8周的C57BL/6J雄性小鼠暴露在含有0.5mgkg(-1)体重(-1)天(-1)ATZ的饮用水中，这是澳大利亚政府使用的无观察到的效果水平，或者是10倍的高剂量(5mgkg(-1)bw(-1)天(-1))。服用低剂量ATZ的小鼠显示总体重和累积体重增加。在12周龄时，两个ATZ暴露组的精子死亡百分率显著增加，而低剂量ATZ组的附睾精子活力下降。在ATZ暴露后，睾丸和肝脏的基因表达也发生了显著的变化。这些数据表明，低剂量的ATZ可以扰乱雄性小鼠的代谢和生殖特征。精子质量的慢性下降和体重增加可能会对男性的生殖能力产生负面影响，进一步的研究应该考虑长期接触ATZ对男性生殖健康的影响。
Atrazine (ATZ) is one of the most widely used herbicides worldwide and is a common contaminant in human drinking water. It disrupts metabolic pathways in plants, and has metabolic and reproductive effects in vertebrates, including humans. Few studies have investigated the effects of exposure to low doses of ATZ, especially during sexual development in males. In this study, we exposed C57BL/6J male mice from weaning for 8 weeks to drinking water containing 0.5mgkg(-1) bodyweight (BW) day(-1) ATZ, the no observed effect' level used by the Australian government, or a 10-fold higher dose (5mgkg(-1) BW day(-1)). Mice treated with the low dose of ATZ showed increased total and cumulative weight gain. At 12 weeks of age, there was a significant increase in the percentage of dead spermatozoa in both ATZ-exposed groups, as well as decreased epididymal sperm motility in the low-dose ATZ group. Significant changes in testis and liver gene expression were also observed following ATZ exposure. These data demonstrate that a low dose of ATZ can perturb metabolic and reproductive characteristics in male mice. A chronic reduction in sperm quality and increased weight gain could have negative consequences on the reproductive capacity of males, and further studies should consider the effects of long-term ATZ exposure on male reproductive health.