A Low-Cost, Rapidly Integrated Debubbler (RID) Module for Microfluidic Cell Culture Applications
A Low-Cost, Rapidly Integrated Debubbler (RID) Module for Microfluidic Cell Culture Applications
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DOI：
10.3390/mi10060360
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发表时间：
2019-06-01
期刊：
影响因子：
3.4
通讯作者：
Abhyankar, Vinay V.
中科院分区：
文献类型：
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作者：
Williams, Matthew J.;Lee, Nicholas K.;Abhyankar, Vinay V.
微流控平台使用受控的流体流动，以明确和可重复的方式向培养细胞提供生理相关的生化和生物物理线索。在这些系统中，不受干扰的流动是至关重要的，进入微流控通道的气泡可能导致器件分层或细胞损坏。为了防止气泡进入微流控通道，我们报告了一种低成本、快速集成的除泡器(RID)模块，该模块制造简单、成本低廉，并且易于与现有的实验系统相结合。我们展示了在对微流体通道中培养的哺乳动物细胞施加生理壁切应力(1-200达因厘米(-2))所需的流速下，成功去除跨越三个数量级的气泡，最大去除速率(dv/dt)(Max)=1.5mLmin(-1)。
Microfluidic platforms use controlled fluid flows to provide physiologically relevant biochemical and biophysical cues to cultured cells in a well-defined and reproducible manner. Undisturbed flows are critical in these systems, and air bubbles entering microfluidic channels can lead to device delamination or cell damage. To prevent bubble entry into microfluidic channels, we report a low-cost, Rapidly Integrated Debubbler (RID) module that is simple to fabricate, inexpensive, and easily combined with existing experimental systems. We demonstrate successful removal of air bubbles spanning three orders of magnitude with a maximum removal rate (dV/dt)(max) = 1.5 mL min(-1), at flow rates required to apply physiological wall shear stress (1-200 dyne cm(-2)) to mammalian cells cultured in microfluidic channels.