Peripheral and central angiotensin II regulates expression of genes of the renin-angiotensin system.
Peripheral and central angiotensin II regulates expression of genes of the renin-angiotensin system.
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外周和中枢血管紧张素 II 调节肾素-血管紧张素系统基因的表达。
DOI：
10.1152/ajpendo.1992.262.5.e651
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Milsted,A
中科院分区：
文献类型：
--
作者：
Kohara,K;Brosnihan,KB;Ferrario,CM;Milsted,A
我们研究了血管紧张素（ANG）II是否具有调节外周和中枢组织中肾素-血管紧张素系统（RAS）基因表达的潜力。通过渗透性微型泵将ANG II（0.1或6.0 nmol/h）静脉内或脑室内输注到雄性Sprague-Dawley大鼠（225-250 g）中，持续5天。我们通过北方印迹分析测量了肝脏、肾上腺和大脑（下丘脑和下脑干）中的血管紧张素原mRNA、肾脏中的血管紧张素转换酶（ACE）mRNA以及肺和睾丸中的血管紧张素转换酶（ACE）mRNA。我们证明，血浆ANG II增加肝血管紧张素原mRNA水平，降低肾肾素mRNA，降低肺ACE mRNA。脑室内施用ANG II导致外周RAS组分的不同反应模式。肝脏血管紧张素原mRNA增加，肾脏肾素mRNA下降，两个剂量的ANG II，而肺ACE mRNA保持无反应。中枢介导的ANG II影响最可能是间接的，因为血浆ANG II浓度没有变化。这项研究表明，ANG II具有影响其形成的调节的深刻的多种作用。此外，结果表明，RAS的基因响应外源性血管紧张素II的组织和途径特异性的方式。
We investigated whether angiotensin (ANG) II has the potential to regulate expression of genes of the renin-angiotensin system (RAS) in peripheral and central tissues. ANG II (0.1 or 6.0 nmol/h) was infused by osmotic minipump into male Sprague-Dawley rats (225-250 g) for 5 days, either intravenously or intracerebroventricularly. We measured angiotensinogen mRNA in liver, adrenal glands, and brain (hypothalamus and lower brain stem), renin mRNA in the kidney, and angiotensin-converting enzyme (ACE) mRNA in the lung and testis by Northern blot analysis. We demonstrated that plasma ANG II increases the levels of liver angiotensinogen mRNA, decreases kidney renin mRNA, and decreases lung ACE mRNA. Intracerebroventricular administration of ANG II resulted in a different pattern of responses of the peripheral RAS components. Liver angiotensinogen mRNA was increased, and kidney renin mRNA was decreased by both doses of ANG II, whereas lung ACE mRNA remained unresponsive at either dose. Centrally mediated influences of ANG II are most likely indirect since plasma ANG II concentration was not changed. This study has revealed that ANG II has profound diverse effects that influence the regulation of its formation. Further, results indicate that genes of the RAS responded to exogenous ANG II in both tissue- and route-specific ways.