Otogelin: A glycoprotein specific to the acellular membranes of the inner ear
Otogelin: A glycoprotein specific to the acellular membranes of the inner ear
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DOI：
10.1073/pnas.94.26.14450
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发表时间：
1997-12-23
影响因子：
11.1
通讯作者：
Petit, C
中科院分区：
文献类型：
--
作者：
Cohen-Salmon, M;El-Amraoui, A;Petit, C
由于哺乳动物内耳的神经上皮细胞数量少，支持细胞种类繁多，因此鉴定内耳特定成分的工作受到阻碍。为了克服这些困难，我们对2日龄小鼠耳蜗的cDNA使用了基于pcr的减法。已经分离出一个预测的含有2910个氨基酸的与粘蛋白相关的cDNA。然而，几条线索的证据表明，该蛋白不经历粘蛋白特征的o -糖基化。免疫组织荧光标记显示，该蛋白是内耳所有脱细胞膜的一个组成部分：即耳蜗的背毡膜、耳小体和耳小体的耳锥体和副膜、半规管的丘，以及先前未描述的覆盖耳小体耳锥体的脱细胞物质。根据其定位，这种蛋白质被命名为otogelin。位于这些膜下的各种非感觉细胞可以被识别为合成otogelin。最后，本研究揭示了被膜的成熟过程，证明了耳胶质标记逐渐组织成厚且间隔的径向纤维样结构。
Efforts to identify the specific components of the mammalian inner ear have been hampered by the small number of neuroepithelial cells and the variety of supporting cells. To circumvent these difficulties, we used a PCR-based subtractive method on cDNA from 2-day-old mouse cochlea. A cDNA encoding a predicted 2910-amino acid protein related to mucin has been isolated. Several lines of evidence indicate, however, that this protein does not undergo the O-glycosylation characteristic to mucins. As confirmed by immunocytochemistry and biochemical experiments, this protein is specific to the inner ear, Immunohistofluorescence labeling showed that this protein is a component of all the acellular membranes of the inner ear: i.e., the tectorial membrane of the cochlea, the otoconial and accessory membranes of the utricule and saccule, the cupula of the semicircular canals, and a previously undescribed acellular material covering the otoconia of the saccule. The protein has been named otogelin with reference to its localization. A variety of nonsensory cells located underneath these membranes could be identified as synthesizing otogelin. Finally, this study revealed a maturation process of the tectorial membrane, as evidenced by the progressive organization of otogelin labeling into thick and spaced radial fiber-like structures.