Purification and structure of the host-specific toxin from Helminthosporium carbonum race 1.
Purification and structure of the host-specific toxin from Helminthosporium carbonum race 1.
复制标题
碳长蠕孢菌 1 号宿主特异性毒素的纯化和结构。
DOI：
10.1016/0006-291x(82)90592-7
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发表时间：
1982
影响因子：
3.1
通讯作者：
Gibson,BW
中科院分区：
文献类型：
--
作者：
Walton,JD;Earle,ED;Gibson,BW
通过溶剂提取、凝胶过滤和高压液相色谱分离，从培养滤液中分离纯化出炭疽病菌1号的寄主特异性毒素。纯化后的毒素经高分辨质谱分析，其相对分子质量为436.2318，元素组成为C21H32N4O6。氨基酸分析、质子和~(13)C-核磁共振谱分析表明，该多肽含有四个氨基酸。用气相色谱-质谱法测定了它们的序列。最后用D-和L-氨基酸氧化酶对氨基酸进行消化，得到完整的结构环[(L-2-氨基-9，10-epoxy-8-oxodecanoyl)-D-prolyl-L-alanyl-L-alanyl].
The host-specific toxin fromHelminthosporiumcarbonumrace 1 was purified from culture filtrates by solvent extraction, gel filtration, and high pressure liquid chromatography. High resolution mass spectrometry of the purified toxin gave a MW of 436.2318 and an elemental composition C21H32N4O6. Amino acid analysis and proton and13C-NMR indicated a peptide containing four amino acids. Their sequence was determined by gas chromatography mass spectrometry. Finally, digestion of the amino acids with D- and L-amino acid oxidases gave the complete structure cyclo[(L-2-amino-9, 10-epoxy-8-oxodecanoyl)-D-prolyl-L-alanyl-L-alanyl].