Macrophages play a key role in early blood brain barrier reformation after hypothermic brain injury
Macrophages play a key role in early blood brain barrier reformation after hypothermic brain injury
复制标题
DOI：
10.1016/j.neulet.2011.06.062
复制
发表时间：
2011-09-01
影响因子：
2.5
通讯作者：
Glod, John
中科院分区：
文献类型：
--
作者：
Koneru, Rajeth;Kobiler, David;Glod, John
中枢神经系统（CNS）创伤性损伤后的炎症反应包括大量巨噬细胞的浸润。这种反应与持续的组织损伤以及中枢神经系统损伤后的恢复有关。我们研究了入侵巨噬细胞在大脑组织修复的一个重要方面——血脑屏障（BBB）的重建中的作用。我们使用脂质体氯膦酸盐来消耗单核细胞和组织巨噬细胞。该方法导致小鼠模型中低温脑损伤部位表达 F4/80 的细胞积累显着减少。通过荧光标记白蛋白的渗透性来评估损伤后血脑屏障随时间的完整性。损伤部位巨噬细胞的减少伴随着血脑屏障早期重建的延迟。在对照动物中，BBB 对 FITC 标记白蛋白的通透性在受伤后 7 天恢复到正常水平。在巨噬细胞耗尽的小鼠中，受伤后 7 天仍观察到白蛋白渗漏。这些结果表明巨噬细胞在血脑屏障创伤后早期重建中发挥着重要作用。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
The inflammatory response following traumatic injury to the central nervous system (CNS) includes the infiltration of large numbers of macrophages. This response has been implicated in both ongoing tissue damage as well as recovery following CNS injury. We investigated the role of invading macrophages on one important aspect of tissue repair in the brain, the reformation of the blood brain barrier (BBB). We used liposomal clodronate to deplete monocytes and tissue macrophages. This method led to a marked reduction in the accumulation of F4/80-expressing cells at sites of hypothermic brain injury in a murine model. The integrity of the blood brain barrier over time following injury was assessed by permeability of fluorescent labeled albumin. The reduction in macrophages at the injury site was accompanied by a delay in early reformation of the blood brain barrier. In control animals the permeability of the BBB to FITC-labeled albumin returned to normal levels by seven days post-injury. In macrophage-depleted mice leakage of albumin was still observed at seven days post-injury. These results suggest that macrophages play an important role in early post-traumatic reformation of the BBB. (C) 2011 Elsevier Ireland Ltd. All rights reserved.