Influences on the reduction of relative telomere length over 10 years in the population-based Bruneck Study: introduction of a well-controlled high-throughput assay
Influences on the reduction of relative telomere length over 10 years in the population-based Bruneck Study: introduction of a well-controlled high-throughput assay
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DOI：
10.1093/ije/dyp273
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发表时间：
2009-12-01
影响因子：
7.7
通讯作者：
Brandstaetter, Anita
中科院分区：
文献类型：
--
作者：
Ehrlenbach, Silvia;Willeit, Peter;Brandstaetter, Anita
方法我们比较了510个样本对的RTL从纵向人群为基础的Bruneck研究，这是在1995年收集，并在2005年重新收集，并额外确定RTL从159名参与者谁在随访期间死亡。RTL测定的高通量实时PCR检测和应用的数学model.Results端粒缩短，平均455 bp，超过10年。RTL缩短率与基线RTL高度相关（r = 0.674，P < 0.001）。在观察期内死亡的参与者的端粒比存活者短得多（中位数RTL为0.98 vs 1.49; P < 0.001）。与以往的研究相比，吸烟行为没有影响RTL和端粒shortening.Conclusion这是第一个全面的纵向研究的个人谁是，平均60在基线，谁被重新评估10年后。我们的方法被证明是一个可靠的工具，快速，准确和具有成本效益的测定RTL与少量的DNA。
Methods We compared RTL in 510 sample pairs from the longitudinal population-based Bruneck Study, which were collected in 1995 and recollected in 2005, and additionally determined RTL from 159 participants who died during follow-up. RTL were determined by a high-throughput real-time PCR assay and by applying a mathematical model.Results The telomeres shortened, on average, by 455 bp over 10 years. The RTL shortening rate was highly correlated with baseline RTL (r = 0.674, P < 0.001). Participants who died within the observed period had considerably shorter telomeres than those who survived (median RTL of 0.98 vs 1.49; P < 0.001). In contrast to previous studies, smoking behaviour had no influence on RTL and on telomere shortening.Conclusion This is the first comprehensive longitudinal study of individuals who were, on average, 60 at baseline, and who were re-evaluated 10 years later. Our methodology proved to be a reliable tool for a rapid, accurate and cost-efficient determination of RTL with a low amount of DNA.