Monoclonal antibodies to human erythrocytes
Monoclonal antibodies to human erythrocytes
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人红细胞单克隆抗体
DOI：
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发表时间：
1982
影响因子：
5.4
通讯作者：
P. Edwards
中科院分区：
文献类型：
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作者：
D. Anstee;P. Edwards
使用一组空型红细胞、酶处理的正常细胞和人红细胞唾液糖蛋白抑制法，检测了8种来自小鼠杂交瘤的抗正常人红细胞单克隆抗体。两种抗体与RhNULL细胞反应不良或根本不反应。这些抗体是相当感兴趣的，因为它可能会使用它们来阐明恒河猴血型系统的抗原的化学性质。其他四种抗体被sialoglycopfotein制剂抑制。识别的抗原分别是主要唾液酸糖蛋白α上的两个不同决定簇（血型糖蛋白A）和一个可能是唾液酸糖蛋白α和δ共有的决定簇（血型糖蛋白A和B）。另一种抗体具有抗-β-内酰胺酶特异性。这些抗体之一是红细胞唾液酸糖蛋白的进一步表征相当大的潜在价值。
Eight monoclonal antibodies from mouse hybridomas raised to normal human erythrocytes were tested with a panel of null‐type erythrocytes, enzyme‐treated normal cells, and by inhibition with human erythrocyte sialoglycoproteins. Two antibodies reacted poorly or not at all with RhNULL cells. These antibodies are of considerable interest since it may be possible to use them to elucidate the chemical nature of the antigens of the Rhesus blood group system. Four other antibodies were inhibited by sialoglycopfotein preparations. The antigens recognized were, respectively, two different determinants on the major sialoglycoprotein α (glycophorin A) and one determinant which is probably common to sialoglycoproteins α and δ (glycophorins A and B). Another antibody had anti‐Wrb specificity. One of these antibodies is of considerable potential value for the further characterization of erythrocyte sialoglycoproteins.