Plasma and Urine Metabolite Profiles Impacted by Increased Dietary Navy Bean Intake in Colorectal Cancer Survivors: A Randomized-Controlled Trial.
Plasma and Urine Metabolite Profiles Impacted by Increased Dietary Navy Bean Intake in Colorectal Cancer Survivors: A Randomized-Controlled Trial.
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DOI：
10.1158/1940-6207.capr-20-0270
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发表时间：
2021-04
期刊：
影响因子：
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通讯作者：
Ryan EP
中科院分区：
文献类型：
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作者：
Zarei I;Baxter BA;Oppel RC;Borresen EC;Brown RJ;Ryan EP
海军豆含有生物活性的植物化学物质，具有预防结肠癌的特性，如致癌物诱导的动物模型所示。人类研究支持膳食海军豆摄入量通过肠道微生物组调节代谢。本研究调查了摄入海军豆对超重和肥胖结直肠癌（CRC）幸存者血浆和尿液代谢产物谱的影响。20名参与者完成了为期4周的单盲、随机对照饮食干预，其中包括预煮海军豆（35克豆粉/天）或对照（0克/天）。在基线、食用后2周和4周收集血浆和尿液。非靶向代谢组学应用于研究膳食和零食、海军豆、血浆和尿液。增加海军豆的消耗被假设为a）描绘饮食生物标志物和B）促进血浆和尿代谢组中与癌症保护相关的代谢变化。在4周时，与安慰剂对照组相比，海军豆干预组中的16种血浆和16种尿液代谢物显著不同（p< 0.05）。血浆2，3-二羟基-2-甲基丁酸盐（1.34倍）、S-甲基半胱氨酸（1.92倍）和哌啶酸盐（3.89倍）以及尿液S-腺苷高半胱氨酸（2.09倍）和半胱氨酸（1.60倍）的增加代表了食用海军豆后具有癌症保护作用的代谢物。在血浆或尿液中检测到饮食衍生的代谢物，并确认其存在于海军豆干预餐和零食中。这些包括3-（4-羟基苯基）丙酸酯、甜菜碱、哌可酸、S-甲基半胱氨酸、胆碱、二十碳五烯酸（20：5 n3）、苯甲酸、S-腺苷高半胱氨酸、N-δ-乙酰鸟氨酸、半胱氨酸、3-（4-羟基苯基）乳酸酯、龙胆酸、马尿酸、4-羟基马尿酸和水杨酸。为期4周的海军豆饮食干预显示了对CRC预防具有重要意义的代谢途径的变化，值得在未来的高风险队列研究中继续关注饮食调节。
Navy beans contain bioactive phytochemicals with colon cancer prevention properties as demonstrated in carcinogen-induced animal models. Human studies support that dietary navy bean intake modulates metabolism by the gut microbiome. This study investigated the effect of navy bean ingestion on plasma and urine metabolite profiles of overweight and obese colorectal cancer (CRC) survivors. Twenty participants completed a single-blinded, randomized-controlled dietary intervention with pre-cooked navy beans (35g bean powder/day) or control (0g/day) for 4 weeks. Plasma and urine were collected at baseline, 2 weeks and 4 weeks following consumption. Non-targeted metabolomics was applied to study meals and snacks, navy beans, plasma, and urine. Increased navy bean consumption was hypothesized to a) delineate dietary biomarkers and b) promote metabolic shifts relevant for cancer protection in the plasma and urine metabolome. At 4 weeks, 16 plasma and 16 urine metabolites were significantly different in the navy bean intervention group compared to placebo-control (p< 0.05). Increased plasma 2,3-dihydroxy-2-methylbutyrate (1.34-fold), S-methylcysteine (1.92-fold), and pipecolate (3.89-fold), and urine S-adenosylhomocysteine (2.09-fold) and cysteine (1.60-fold) represent metabolites with cancer protective actions following navy bean consumption. Diet-derived metabolites were detected in plasma or urine and confirmed for presence in the navy bean intervention meals and snacks. These included 3-(4-hydroxyphenyl)propionate, betaine, pipecolate, S-methylcysteine, choline, eicosapentaenoate (20:5n3), benzoate, S-adenosylhomocysteine, N-delta-acetylornithine, cysteine, 3-(4-hydroxyphenyl)lactate, gentisate, hippurate, 4-hydroxyhippurate, and salicylate. The navy bean dietary intervention for 4 weeks showed changes to pathways of metabolic importance to CRC prevention and merit continued attention for dietary modulation in future high-risk cohort investigations.