INTERLEUKIN-10 IS AN AUTOCRINE GROWTH-FACTOR FOR ACQUIRED IMMUNODEFICIENCY SYNDROME-RELATED B-CELL LYMPHOMA
INTERLEUKIN-10 IS AN AUTOCRINE GROWTH-FACTOR FOR ACQUIRED IMMUNODEFICIENCY SYNDROME-RELATED B-CELL LYMPHOMA
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DOI：
10.1182/blood.v85.12.3423.bloodjournal85123423
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发表时间：
1995-06-15
期刊：
影响因子：
20.3
通讯作者：
GILL, PS
中科院分区：
文献类型：
--
作者：
MASOOD, R;ZHANG, Y;GILL, PS
白细胞介素-10（IL-10）是一种分子量为35 kD的酸敏感蛋白，具有多种效应，包括抑制细胞毒性T细胞反应、诱导B淋巴细胞中的主要组织相容性复合物II型、诱导B细胞生长和分化以及单核细胞中的自分泌生长因子活性。我们和其他人已经表明，IL-10是由人类免疫缺陷病毒血清阳性患者的血液单核细胞自发产生的。为了确定IL-10在获得性免疫缺陷综合征（AIDS）相关的B细胞淋巴瘤中的潜在作用，我们评估了人IL-10在肿瘤衍生的B细胞系和原发性肿瘤细胞中的表达。在检测的5个细胞系中的4个中检测到人IL-10（hIL-10）mRNA和蛋白的表达。IL-10反义寡核苷酸抑制IL-10 mRNA表达和IL-10蛋白产生。所有B细胞系的增殖均被反义寡核苷酸以剂量依赖性方式抑制，该方式通过添加重组hIL-10蛋白而被废除。在不产生hIL-10的B细胞系中未观察到反义寡核苷酸的作用。对来自患有AIDS-淋巴瘤细胞的患者的原发性肿瘤细胞的评估显示出对IL-10的类似的产生和应答。这些数据表明，自分泌生长机制的IL-10在艾滋病相关的淋巴瘤细胞和IL-10可能是重要的，在其发病机制。(C)1995年，美国血液学会。
Interleukin-10 (IL-10) is an acid-sensitive protein of 35 kD that has pleiotropic effects including inhibition of cytotoxic T-cell response, induction of major histocompatibility complex type II in B lymphocytes, induction of B-cell growth and differentiation, and autocrine growth factor activity in monocytes. We and others have shown that IL-10 is produced spontaneously by blood mononuclear cells from human immunodeficiency virus-seropositive patients. In an attempt to ascertain the potential role of IL-10 in acquired immunodeficiency syndrome (AIDS)-related B-cell lymphoma, we evaluated the expression of human IL-10 in both tumor-derived B-cell lines and primary tumor cells. Expression of human IL-10 (hIL-10) mRNA and protein was detected in four of five cell lines examined. An IL-10 antisense oligonucleotide inhibited IL-10 mRNA expression and IL-10 protein production. The proliferation of all B-cell lines was inhibited by an antisense oligonucleotide in a dose-dependent manner that was abrogated by the addition of recombi- nant hIL-10 protein. No effect of antisense oligonucleotide was observed in the B-cell line not producing hIL-10. Evaluation of primary tumor cells from patients with AIDS-lymphoma cells showed similar production and response to IL-10. These data suggest an autocrine growth mechanism for IL-10 in AIDS-related lymphoma cells and that IL-10 may be important in its pathogenesis. (C) 1995 by The American Society of Hematology.