EXTRACTION OF DNA FROM MILLIGRAM AMOUNTS OF FRESH, HERBARIUM AND MUMMIFIED PLANT-TISSUES
EXTRACTION OF DNA FROM MILLIGRAM AMOUNTS OF FRESH, HERBARIUM AND MUMMIFIED PLANT-TISSUES
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DOI：
10.1007/bf00020088
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发表时间：
1985-01-01
影响因子：
5.1
通讯作者：
BENDICH, AJ
中科院分区：
文献类型：
--
作者：
ROGERS, SO;BENDICH, AJ
我们已经开发了一种毫克量的植物组织的DNA提取程序。产量范围为每毫克组织0.3-200纳克DNA。讨论了影响产率的因素。使用了新鲜的组织，以及植物标本（22-118岁）和木乃伊化的种子和胚胎（500至超过44，600岁）。所有尝试的组织（来自29个物种的57种）都产生了可测量的DNA量。在测试的情况下，没有观察到限制性酶BamHI或EcoRI的抑制。
We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to greater than 44,600 years old) were used. All tissues attempted (57 types from 29 species) yielded measurable amounts of DNA. In no case tested was inhibition observed for restriction enzymes BamHI or EcoRI.