Stem cell regulatory function mediated by expression of a novel mouse Oct4 pseudogene
Stem cell regulatory function mediated by expression of a novel mouse Oct4 pseudogene
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DOI：
10.1016/j.bbrc.2007.01.106
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发表时间：
2007-03-30
影响因子：
3.1
通讯作者：
Lee, Techung
中科院分区：
文献类型：
--
作者：
Lin, Huey;Shabbir, Arsalan;Lee, Techung
已经提出了多个假基因的胚胎干（ES）细胞特异性基因，它们的丰度表明，这些潜在的假基因可能是功能性的。胚胎干细胞特异性表达Oct4调控干细胞多能性和自我更新状态虽然Oct4在基因重编程过程中在成人组织中表达，但检测到的Oct4信号可能是由Oct4假基因贡献的。在这里表征的多个Oct4转录物中，是来自P19胚胎癌干细胞的类似于1kb的克隆，其与亲本Oct4基因共享类似于87%的序列同源性，并且具有编码80个氨基酸产物（命名为Oct4P1）的潜力。Oct4P1在间充质干细胞中的腺病毒表达促进其增殖并抑制其骨软骨分化。Oct4P1的这些双重作用使人联想到亲本Oct4的干细胞调节功能，并表明Oct4PI可能是一个功能性假基因或在干细胞中具有独特功能的新型Oct4相关基因。(c)2007年爱思唯尔公司All rights reserved.
Multiple pseudogenes have been proposed for embryonic stem (ES) cell-specific genes, and their abundance suggests that some of these potential pseudogenes may be functional. ES cell-specific expression of Oct4 regulates stem cell pluripotency and self-renewing state. Although Oct4 expression has been reported in adult tissues during gene reprogramming, the detected Oct4 signal might be contributed by Oct4 pseudogenes. Among the multiple Oct4 transcripts characterized here is a similar to 1 kb clone derived from P19 embryonal carcinoma stem cells, which shares a similar to 87% sequence homology with the parent Oct4 gene, and has the potential of encoding an 80-amino acid product (designated as Oct4P1). Adenoviral expression of Oct4P1 in mesenchymal stem cells promotes their proliferation and inhibits their osteochondral differentiation. These dual effects of Oct4P1 are reminiscent of the stem cell regulatory function of the parent Oct4, and suggest that Oct4PI may be a functional pseudogene or a novel Oct4-related gene with a unique function in stem cells. (c) 2007 Elsevier Inc. All rights reserved.