A molecular aspect of symbiotic interactions between the weevil Sitophilus oryzae and its endosymbiotic bacteria: Over-expression of a chaperonin
A molecular aspect of symbiotic interactions between the weevil Sitophilus oryzae and its endosymbiotic bacteria: Over-expression of a chaperonin
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DOI：
10.1006/bbrc.1997.7552
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发表时间：
1997-10-29
影响因子：
3.1
通讯作者：
Nardon, P
中科院分区：
文献类型：
--
作者：
Charles, H;Heddi, A;Nardon, P
通过双向电泳蛋白质图谱的比较，分析了象甲Sitophilus sativa共生和非共生菌株的共生特异蛋白质。一种蛋白质被证明是专门表达的脱共生菌株和三种蛋白质，包括伴侣蛋白，其特征在于在共生菌株模式。groE样操纵子，编码两个伴侣蛋白groES和GroEL样蛋白的内吞生物，进行测序。发现它与大肠杆菌的groE操纵子非常相似（82%同一性）。蛋白质标记的体外和离体实验表明，近40%的离体内吞生物体蛋白质合成集中在GroEL样蛋白上。最后，我们用北方印迹法证明，38 ℃热休克导致groEL mRNA在内吞生物体内积累，这项工作支持了伴侣蛋白在维持共生联合中具有重要生理功能的假设。(C)1997年学术出版社。
Specific proteins of symbiosis were analyzed by the comparison of two-dimensional electrophoresis protein patterns of symbiotic and aposymbiotic strains of the weevil Sitophilus oryzae. One protein was shown to be exclusively expressed in the aposymbiotic strain and three proteins, including a chaperonin, were characterized in the symbiotic strain pattern. The groE-like operon, encoding the two chaperonins groES and GroEL-like proteins of the endocytobiotes, was sequenced. It was found to be very similar to the groE operon of Escherichia coli (82% identity). In vitro and ex vivo experiments of protein labelling demonstrated that almost 40% of the endocytobiote protein synthesis ex vivo is focused on the GroEL-like protein. Finally, we showed by northern blotting that heat shock at 38 degrees C results in groEL mRNA accumulation inside the endocytobiotes, This work supports the hypothesis that chaperonins could have an essential physiological function in the maintenance of the symbiotic association. (C) 1997 Academic Press.