In vitro cytotoxicity of five glass-ionomer cements
In vitro cytotoxicity of five glass-ionomer cements
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DOI：
10.1016/s0142-9612(03)00253-9
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发表时间：
2003-09-01
期刊：
影响因子：
14
通讯作者：
Hanks, CT
中科院分区：
文献类型：
--
作者：
Costa, CAD;Hebling, J;Hanks, CT
为评价5种玻璃离子水门汀对人成牙本质细胞系MDPC-23的细胞毒作用，将5种玻璃离子水门汀分别制成玻璃体粘结剂1组、玻璃体粘固剂2组、富士IIlC组、富士IX GP组、Ketac-Molar组、Z-100阳性对照组。第7组在滤纸上涂抹磷酸盐缓冲盐水(阴性对照)。将标本放置于孔底后，将30,000个/cm2的细胞接种培养72 h，计数细胞数，扫描电子显微镜观察细胞形态，甲基四氮唑蓝比色法测定细胞代谢。Kruskal-Wallis的统计分析用于确定细胞代谢和细胞数量在95%可信水平上是否有差异。第1、2、3、4、5、6组细胞数分别减少74.5%、75.5%、45.5%、29.5%、32.5%、88.5%。在第1、2、3、4和5组，实验性GICs使细胞代谢分别降低79%、84%、54%、40%和42.5%。尽管所有实验材料对MDPC-23细胞都有细胞毒性，但GICs的细胞毒性最小。另一方面，RMGIC对细胞的吞噬作用最强。(C)2003爱思唯尔科学有限公司。保留所有权利。
To evaluate the cytotoxic effects of five glass-ionomer cements (GICs) on an odontoblast cell line (MDPC-23), disks of every material were prepared and divided into Group 1: Vitrebond, Group 2: Vitremer, Group 3: Fuji IILC, Group 4: Fuji IX GP, Group 5: Ketac-Molar, Group 6: Z-100 (positive control). In Group 7, phosphate-buffered saline solution (negative control) was applied on filter paper. After placing the samples in the bottom of wells, the cells (30,000 cells/cm(2)) were plated and incubated for 72 h. The cell number was counted, the cell morphology was assessed by scanning electron microscopy and the cell metabolism was evaluated using methyltetrazolium assay. The statistical analysis of Kruskal-Wallis was used to determine if the scores obtained for the cell metabolism and number of cells were different at the 95% confidence level. In groups 1, 2, 3, 4, 5, and 6 the materials decreased the cell number by 74.5% 75.5%, 45.5%, 29.5%, 32.5%, and 88.5%, respectively. In groups 1, 2, 3, 4, and 5, the experimental GICs reduced the cell metabolism by 79%, 84%, 54%, 40%, and 42.5%, respectively. Despite the fact that all experimental materials were cytotoxic to the MDPC-23 cells, the GICs were the least cytotoxic. On the other hand, the RMGICs caused the highest cytophatic effects. (C) 2003 Elsevier Science Ltd. All rights reserved.