The complete mitochondrial genome of the large yellow croaker, Larimichthys crocea (Perciformes, Sciaenidae): Unusual features of its control region and the phylogenetic position of the Sciaenidae
The complete mitochondrial genome of the large yellow croaker, Larimichthys crocea (Perciformes, Sciaenidae): Unusual features of its control region and the phylogenetic position of the Sciaenidae
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DOI：
10.1016/j.gene.2008.11.024
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发表时间：
2009-03-01
期刊：
影响因子：
3.5
通讯作者：
Chu, Ka Hou
中科院分区：
文献类型：
--
作者：
Cui, Zhaoxia;Liu, Yuan;Chu, Ka Hou
为了了解大黄鱼在鲈亚目中的系统地位，我们采用454测序合成技术测定了大黄鱼线粒体基因组全序列。线粒体基因组全长16,466 bp，包括22个tRNA、2个rRNA、13个蛋白质编码基因和非编码控制区（noncoding control region，CR）。使用引物Cyt b-F和12 S-R对6个大黄鱼个体和2个多聚乳酸乳杆菌个体进行完全CR的进一步测序。有趣的是，来自大黄鱼的所有7个CR序列是相同的，而来自多活性乳杆菌的3个序列是不同的（包括来自GenBank的一个）。虽然TAS和CSB-1、CSB-2和CSB-3等保守区在这两种鱼的控制区中很容易被识别，但在石首鱼科的尖尾犬牙石首鱼和其它鲈亚目鱼中却没有检测到典型的中央保守区CSB-D、-E和-F。系统发育分析表明，大黄鱼是石首鱼科中出现较晚的一个种，该科与鲈亚目中的鱼棘科关系密切。大黄鱼作为石首鱼科第一个获得完整线粒体基因组的物种，将为该类群的分子进化提供重要信息。此外，本研究设计的一对特异性引物扩增出了完整的线粒体控制区，为Larimichthys的种群遗传学和保护生物学研究提供了重要依据。(c)2008 Elsevier B. V.保留所有权利。
To understand the systematic status of Larimichthys crocea in the Percoidei, we determined the complete mitochondrial (mt) genome sequence using 454 sequencing-by-synthesis technology. The complete mt genome is 16,466 bp in length including the typical structure of 22 tRNAs, 2 rRNAs, 13 protein-coding genes and the noncoding control region (CR). Further sequencing for the complete CR was performed using the primers Cyt b-F and 12S-R on six L crocea individuals and two L polyactis individuals. Interestingly, all seven CR sequences from L crocea were identical while the three sequences from L polyactis were distinct (including one from GenBank). Although the conserved blocks such as TAS and CSB-1, -2, and -3 are readily identifiable in the control regions of the two species, the typical central conserved blocks CSB-D, -E, and -F could not be detected, while they are found in Cynoscion acoupa of Sciaenidae and other Percoidei species. Phylogenetic analysis shows that L crocea is a relatively recently emerged species in Sciaenidae and this family is closely related to family Pomacanthidae within the Percoidei. L crocea, as the first species of Sciaenidae with complete mitochondrial genome available, will provide important information on the molecular evolution of the group. Moreover, the genus-specific pair of primers designed in this study for amplifying the complete mt control region will be very useful in studies on the population genetics and conservation biology of Larimichthys. (c) 2008 Elsevier B.V. All rights reserved.