Simian immunodeficiency virus infection in a patas monkey (Erythrocebus patas): Evidence for cross-species transmission from African green monkeys (Cercopithecus aethiops sabaeus) in the wild
Simian immunodeficiency virus infection in a patas monkey (Erythrocebus patas): Evidence for cross-species transmission from African green monkeys (Cercopithecus aethiops sabaeus) in the wild
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DOI：
10.1099/0022-1317-77-4-773
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发表时间：
1996-04-01
影响因子：
3.8
通讯作者：
Veas, F
中科院分区：
文献类型：
--
作者：
BibolletRuche, F;GalatLuong, A;Veas, F
对塞内加尔非洲绿猴（Cercopithecus aethiops sabaeus）和赤猴（Erythrocebus patas）种群的社会行为学研究记录了这两个物种之间的身体接触。猴免疫缺陷病毒（SIV）血清阳性率在不同的AGM亚种中有升高的报道。我们在这里报告了猴被感染的程度，并比较了从这两个不同物种分离的病毒的亲缘关系。在研究的85只AGMs和54只patas猴子中，分别有47%和7.5%的抗体与HIV-2包膜蛋白发生交叉反应。从两个agm中分离出一种病毒。从猕猴身上通常无法分离出病毒，但从一只患病的动物身上，用PCR扩增出一种名为pamG31的病毒。此外，对于两个SIVagm分离株，扩增了横跨em和nef基因的830 bp区域并进行了测序。env/nef区的序列比较显示，来自沙巴属亚种AGMs的pamG31和SIVagm分离株的同源性为80%，两个SIVagm分离株的同源性为94%。系统发育分析表明，pamG31属于SIVagm sabaeus亚群。这是首例慢病毒感染猕猴的报道。pamG31和SIVagm沙巴氏病毒之间的密切遗传关系是支持SIV在野生agm和patas猴之间跨物种传播的有力论据。基于这些原因，我们建议将此patas病毒命名为SIVagm-pamG31。
Socio-ethological studies on troops of African green monkeys (AGMs) (Cercopithecus aethiops sabaeus) and patas monkeys (Erythrocebus patas) in Senegal have documented physical contacts between these two species. Elevated simian immunodeficiency virus (SIV) seroprevalence rates have been reported for the different AGM subspecies. We report here the extent to which patas monkeys are infected and compare the relatedness of the viruses isolated from these two different species. Among the 85 AGMs and 54 patas monkeys studied, 47% and 7.5%, respectively, had antibodies that crossreacted with HIV-2 envelope proteins. From two AGMs a virus was isolated. From the patas monkeys, virus isolation was generally not possible, but from one animal that was ill a virus designated pamG31 was amplified by PCR. In addition, for the two SIVagm isolates, an 830 bp region spanning the em, and nef genes was amplified and sequenced. Comparisons of sequences from the env/nef region revealed 80% identity between pamG31 and SIVagm isolates from AGMs of the sabaeus subspecies, and 94% identity between the two SIVagm isolates. Phylogenetic analysis showed that pamG31 belongs to the SIVagm sabaeus subgroup. This is the fist report of a lentiviral infection in a patas monkey. The close genetic relatedness between pamG31 and SIVagm sabaeus viruses is a strong argument in favour of cross-species transmission of SIV between AGMs and patas monkeys in the wild. For these reasons, we propose to refer to this patas virus as SIVagm-pamG31.