Reduction of linoleic acid inhibition in production of conjugated linoleic acid by Propionibacterium freudenreichii ssp. shermanii.
Reduction of linoleic acid inhibition in production of conjugated linoleic acid by Propionibacterium freudenreichii ssp. shermanii.
复制标题
费氏丙酸杆菌亚种生产共轭亚油酸时亚油酸抑制的减少。
DOI：
10.1139/w01-073
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发表时间：
2001
影响因子：
2.8
通讯作者：
S. Laakso
中科院分区：
文献类型：
--
作者：
A. Rainio;M. Vahvaselkä;Tarja Suomalainen;S. Laakso
一种利用弗氏丙酸杆菌SSP培养的亚油酸(LA)生产共轭亚油酸(CLA)的方法。Shermanii JS是开发出来的。通过将LA分散在足够浓度的聚氧乙烯山梨醇单油酸酯洗涤剂中，消除了LA的生长抑制作用。对于乳清渗透介质，乳清与洗涤剂的最佳比例为1：15(w/w)。结果，培养物至少能耐受1000微克×毫升(-1)的乳酸，转化为共轭亚油酸的效率为57%-87%。顺式-9、反式-11和反式-9、顺式-11异构体占共轭亚油酸产量的85%~90%。在De Man Rogosa-Sharpe(MRS)肉汤中也证明了该方法的可行性。
A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate detergent. For the whey permeate medium used, the optimum LA:detergent ratio was 1:15 (w/w). As a result, the cultures tolerated at least 1000 microg x mL(-1) LA, which was converted to CLA with 57%-87% efficiency. The cis-9, trans-11 and trans-9, cis-11 isomers constituted 85%-90% of the CLA produced. The feasibility of the method was demonstrated also in de Man Rogosa-Sharpe (MRS) broth.