Electrokinetic chromatography using thermodynamically stable vesicles and mixed micelles formed from oppositely charged surfactants
Electrokinetic chromatography using thermodynamically stable vesicles and mixed micelles formed from oppositely charged surfactants
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DOI：
10.1021/ac970730y
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发表时间：
1998-04-01
影响因子：
7.4
通讯作者：
Foley, JP
中科院分区：
文献类型：
--
作者：
Hong, M;Weekley, BS;Foley, JP
研究了由带相反电荷的表面活性剂、十二烷基硫酸钠（SDS）和正十二烷基三甲基溴化铵（DTAB）组成的新型混合表面活性剂体系的电动色谱（EKC）。研究了由混合物形成的大脂质体状自发囊泡和棒状混合胶束的色谱特征，并与 SDS 胶束的色谱特征进行了比较。一系列 12-烷基苯酮的分离表明，自发囊泡提供的洗脱窗口比 SDS 胶束宽约 2 倍。发现囊泡和混合胶束系统都比 SDS 提供更大的亚甲基选择性。一组硝基甲苯几何异构体与 DTAB/SDS 自发囊泡和 SDS 胶束假固定相的不同洗脱顺序表明这两个系统可能存在不同的分离机制。对囊泡、混合胶束和 SDS 胶束之间的极性基团选择性、保留和效率进行了比较。还使用 DTAB/SDS 囊泡研究了 20 种中性化合物的保留因子对数 (log k') 和辛醇-水分配系数 (log P-ow) 之间的相关性。自发囊泡作为动电色谱中的伪固定相具有巨大的潜力。
The electrokinetic chromatography (EKC) of a novel mixed surfactant system consisting of oppositely charged surfactants, sodium dodecyl sulfate (SDS) and n-dodecyltrimethylammonium bromide (DTAB), was investigated. The chromatographic characteristics of large liposome-like spontaneous vesicles and rodlike mixed micelles formed from the mixture were explored and compared with those of SDS micelles. Separations of a series of 12-alkylphenones showed that the spontaneous vesicles provided about a 2 times wider elution window than SDS micelles. Both vesicle and mixed micelle systems were found to provide larger methylene selectivity than SDS. The different elution order of a group of nitrotoluene geometric isomers with DTAB/SDS spontaneous vesicles and SDS micelles pseudostationary phases suggested the possibility of different separation mechanisms with these two systems. Comparisons of polar group selectivity, retention, and efficiency were made between vesicles, mixed micelles, and SDS micelles. The correlation between the logarithms of the retention factors (log k') and octanol-water partition coefficients (log P-ow) for a group of 20 neutral compounds was also studied with DTAB/SDS vesicles. Spontaneous vesicles have great potential as a pseudostationary phase in electrokinetic chromatography.