In Vivo Effects of β‐Bungarotoxin on the Acetylcholine System in Different Brain Areas of the Rat
In Vivo Effects of β‐Bungarotoxin on the Acetylcholine System in Different Brain Areas of the Rat
复制标题
β-金环蛇毒素对大鼠不同脑区乙酰胆碱系统的体内影响
DOI：
10.1111/j.1471-4159.1984.tb06685.x
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发表时间：
1984
影响因子：
4.7
通讯作者：
Z. Rakonczay
中科院分区：
文献类型：
--
作者：
K. Gulya;D. Budai;Péter Kása;Z. Rakonczay
摘要：本实验研究了β-银环蛇毒素（β-bungarotoxin，β-BT）对大鼠全脑及不同脑区乙酰胆碱（acetylcholine，ACh）系统的影响。β-BT对脑ACh和胆碱（Ch）含量的影响呈时间依赖性。结果表明，单次微量脑室注射1 μg毒素可增加皮质、海马和小脑中ACh和Ch含量，而纹状体中ACh水平降低。在第一天和第三天两次向侧脑室注射10纳克毒素，导致最后一次治疗后2天ACh水平降低。在第一、第三和第五天用相同剂量处理三次并在最后一次注射后2天处死的动物中，胆碱乙酰转移酶和乙酰胆碱酯酶活性降低，毒蕈碱乙酰胆碱受体数量减少。因此证明了毒素的双相效应。结果表明，在毒素作用的第一阶段，ACh和Ch水平的升高可能是由于抑制神经元的传递，而在第二阶段，当ACh系统的成分减少时，β-BT的神经元变性作用起着重要作用。
Abstract: The in vivo effects of β‐bungarotoxin (β‐BT) on the acetylcholine (ACh) system were studied in the whole cerebrum and in different brain regions. The effect of β‐BT on cerebral ACh and choline (Ch) contents was time‐dependent. The results show that a singe intrace‐ rebroventricular injection of 1 μg toxin increased both the ACh and Ch contents in the cortex, hippocampus, and cerebellum, while in the striatum the ACh level was decreased. Ten nanograms of toxin injected into the lateral ventricle twice, on the first and third days, led to a reduced ACh level 2 days after the last treatment. In animals treated with the same dose three times, on the first, third, and fifth days, and sacrificed 2 days after the last injection, the choline acetyltransferase and acetylcholinesterase activities were reduced and the number of muscarinic acetylcholine receptors was decreased. A biphasic effect of the toxin was therefore demonstrated. It is suggested that in the first phase of the toxin effect the increased levels of ACh and Ch may be due to the inhibition of neuronal transmission, while in the second phase, when the elements of the ACh system are reduced, the neuronal degenerating effect of β‐BT plays a significant role.