Role of corticotropin-releasing factor on bladder function in rats with psychological stress
Role of corticotropin-releasing factor on bladder function in rats with psychological stress
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DOI：
10.1038/s41598-019-46267-9
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发表时间：
2019-07-08
影响因子：
4.6
通讯作者：
Yokoyama, Osamu
中科院分区：
文献类型：
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作者：
Seki, Masaya;Zha, Xin-Min;Yokoyama, Osamu
应激相关肽促肾上腺皮质激素释放因子（CRF）和CRF相关肽分布于膀胱等外周脏器。我们调查了心理应激（PS）和CRF对膀胱功能的贡献。雄性大鼠接受假应激（SS）或PS使用通信箱的方法，每天120分钟，共7天。一组大鼠在应激暴露期间接受腹膜内CRF-R1拮抗剂antalarmin 7天。PS大鼠每次排尿的平均排尿量显著低于SS大鼠，这被Antalarmin治疗所拮抗。血浆和膀胱CRF，膀胱CRF，CRF-R1和M2/3毒蕈碱受体的mRNA表达增加，发现在PS大鼠。CRF本身不影响膀胱收缩，但应激可增加膀胱肌条的毒蕈碱收缩反应。这些变化被antalarmin治疗所拮抗。总之，PS通过CRF-R1加强M3受体介导的收缩，导致膀胱储存功能障碍。
Stress-related peptide corticotropin-releasing factor (CRF) and CRF-related peptides are distributed in the peripheral viscera such as the bladder. We investigated the contribution of psychological stress (PS) and CRF on bladder function. Male rats received sham stress (SS) or PS using a communication box method for 120 min every day for 7 days. One group of rats received the intraperitoneal CRF-R1 antagonist antalarmin for 7 days during stress exposure. Mean voided volume per micturition was significantly lower in PS rats compared to SS rats, which was antagonized by antalarmin treatment. Increases in plasma and bladder CRF, and mRNA expressions of bladder CRF, CRF-R1, and M2/3 muscarinic receptors, were found in PS rats. CRF did not influence bladder contraction in itself; however, stress increased the response of muscarinic contraction of bladder strips. These changes were antagonized by antalarmin treatment. In conclusion, PS reinforces M3 receptor-mediated contractions via CRF-R1, resulting in bladder storage dysfunction.