SPECIFIC RECOGNITION OF CRUCIFORM DNA BY NUCLEAR-PROTEIN HMG1
SPECIFIC RECOGNITION OF CRUCIFORM DNA BY NUCLEAR-PROTEIN HMG1
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DOI：
10.1126/science.2922595
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发表时间：
1989-02-24
期刊：
影响因子：
56.9
通讯作者：
PAONESSA, G
中科院分区：
文献类型：
--
作者：
BIANCHI, ME;BELTRAME, M;PAONESSA, G
十字形DNA是一种非双螺旋形式的DNA，可以作为基因重组的中间体以及在超螺旋作用下从回文序列产生。真核细胞配备有 DNA 结合蛋白，可以选择性识别十字形 DNA。生化和免疫学数据表明，这种蛋白质是 HMG1，是细胞核中进化保守、必需且丰富的成分。与普遍存在的蛋白质的相互作用表明十字形 DNA 构象的关键作用。
Cruciform DNA, a non-double helix form of DNA, can be generated as an intermediate in genetic recombination as well as from palindromic sequences under the effect of supercoiling. Eukaryotic cells are equipped with a DNA-binding protein that selectively recognizes cruciform DNA. Biochemical and immunological data showed that this protein is HMG1, an evolutionarily conserved, essential, and abundant component of the nucleus. The interaction with a ubiquitous protein points to a critical role for cruciform DNA conformations.