Inhibition of binding of Helicobacter pylori to the glycolipid receptors by probiotic Lactobacillus reuteri
Inhibition of binding of Helicobacter pylori to the glycolipid receptors by probiotic Lactobacillus reuteri
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DOI：
10.1111/j.1574-695x.2002.tb00541.x
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发表时间：
2002-01-14
影响因子：
--
通讯作者：
Ohori, H
中科院分区：
文献类型：
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作者：
Mukai, T;Asasaka, T;Ohori, H
我们研究了罗伊氏乳杆菌和幽门螺杆菌与神经节四糖神经酰胺（去唾液酸-GM 1）和硫苷脂（H. pylori中的一种可能的硫苷脂结合蛋白。罗伊氏菌株。在9个L. reuteri菌株中，两个菌株（JCM 1081和TM 105）显示与去唾液酸-GM 1和硫苷脂结合，并抑制H. pylori对两种糖脂的影响。菌株TM 105的细菌细胞提取物与几种去污剂，包括辛基β-D-吡喃葡萄糖苷，保持与两种糖脂的结合，并抑制H。pylori结合，表明结合抑制剂与细菌细胞表面相关。当细胞提取物被施加到琼脂糖凝胶固定半乳糖3-硫酸盐对应于硫苷脂的糖部分的结构，一个约47-kDa的蛋白质被发现结合到凝胶。这一观察结果有力地表明，选择L。reuteri菌株有助于在H. pylori，并提出L. reuteri菌株与H.幽门螺杆菌具有作为益生菌的潜力。(C)2002年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
We examined the competition of binding of Lactobacillus reuteri and Helicobacter pylori to gangliotetraosylceramide (asialo-GM1) and sulfatide which are putative glycolipid receptor molecules of H. pylori, and identified a possible sulfatide-binding protein of the L. reuteri strain. Among nine L. reuteri strains, two (JCM1081 and TM105) were shown to bind to asialo-GM1 and sulfatide, and to inhibit binding of H. pylori to both glycolipids by a thin layer chromatogram-overlay assay using biotin-labeled bacterial cells. The extract from the bacterial cells of strain TM105 with several detergents, including octyl beta-D-glucopyranoside, retained binding to both glycolipids and also inhibited H. pylori binding, suggesting that a binding inhibitor(s) is associated with the bacterial cell surface. When the cell extract was applied to the agarose gel immobilized galactose 3-sulfate corresponding to the structure of sugar moieties of sulfatide, an approximately 47-kDa protein was found to bind to the gel. This observation strongly suggested that inhibition by selected L. reuteri strains help to prevent infection in an early stage of colonization in H. pylori and proposed that L. reuteri strains sharing glycolipid specificity with H. pylori have a potential as probiotics. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.