Dose-dependent and cell type-specific cell death and proliferation following in vitro exposure to radial extracorporeal shock waves.
Dose-dependent and cell type-specific cell death and proliferation following in vitro exposure to radial extracorporeal shock waves.
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DOI：
10.1038/srep30637
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发表时间：
2016-08-01
影响因子：
4.6
通讯作者：
Schmitz C
中科院分区：
文献类型：
--
作者：
Hochstrasser T;Frank HG;Schmitz C
径向体外冲击波（rESW）治疗广泛用于肌肉骨骼疾病和伤口修复。然而，其作用机制在很大程度上仍然未知。目前的研究比较了rESW对两种细胞类型的影响。将人胎儿包皮成纤维细胞（HFFF 2）和人胎盘绒毛膜癌细胞系JEG-3暴露于用Swiss DolorClast装置（2.5巴，1 Hz）产生的0、100、200、500或5000个rESW。rESW暴露后立即进行的FACS分析显示，最初，rESW诱导的是机械性细胞破坏，而不是调节性或程序性细胞死亡。细胞损伤几乎被否定，减少空化。此外，随着rESW数量的增加，细胞活力逐渐降低。暴露于rESW对JEG-3细胞的生长潜力没有影响，但剂量依赖性地增加HFFF 2细胞的生长潜力。最初暴露于条件培养基中的假rESW的细胞培养增加了HFFF 2细胞的生长潜力，然而，通过直接暴露于rESW实现了更强的效果。此外，细胞周期分布分析表明，在HFFF 2细胞中从G 0/G1到G2/M期的比例发生了变化，但在JEG-3细胞中没有发生变化。这些数据表明，rESW导致初始和随后的剂量依赖性和细胞类型特异性体外效应。
Radial extracorporeal shock wave (rESW) therapy is widely used in musculoskeletal disorders and wound repair. However, the mechanisms of action are still largely unknown. The current study compared the effects of rESWs on two cell types. Human fetal foreskin fibroblasts (HFFF2) and human placental choriocarcinoma cell line JEG-3 were exposed to 0, 100, 200, 500 or 5000 rESWs generated with a Swiss DolorClast device (2.5 bar, 1 Hz). FACS analysis immediately after rESW exposure showed that initially, rESWs rather induced mechanical cell destruction than regulated or programmed cell death. Cell damage was nearly negated by reducing cavitation. Furthermore, cell viability decreased progressively with higher numbers of rESWs. Exposure to rESWs had no impact on growth potential of JEG-3 cells, but dose-dependently increased growth potential of HFFF2 cells. Cultivation of cells that were initially exposed to sham-rESWs in conditioned media increased the growth potential of HFFF2 cells, nevertheless, an even stronger effect was achieved by direct exposure to rESWs. Additionally, cell cycle distribution analysis demonstrated a shift in proportion from G0/G1 to G2/M phase in HFFF2 cells, but not in JEG-3 cells. These data demonstrate that rESWs leads to initial and subsequent dose-dependent and cell type-specific effects in vitro.