EFFECTS OF SULFATE CONCENTRATION, ELECTROENDOSMOTIC FLOW, AND ELECTRICAL-RESISTANCE OF AGARS AND AGAROSES ON COUNTERIMMUNOELECTROPHORESIS WITH ADENOVIRUS ANTIGENS AND ANTISERA
EFFECTS OF SULFATE CONCENTRATION, ELECTROENDOSMOTIC FLOW, AND ELECTRICAL-RESISTANCE OF AGARS AND AGAROSES ON COUNTERIMMUNOELECTROPHORESIS WITH ADENOVIRUS ANTIGENS AND ANTISERA
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DOI：
10.1016/0022-1759(76)90019-3
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发表时间：
1976-01-01
影响因子：
2.2
通讯作者：
HIERHOLZER, JC
中科院分区：
文献类型：
--
作者：
HIERHOLZER, JC
采用标准技术测定了23种常用琼脂和琼脂糖的硫酸浓度、电内渗（EE）和电阻，并与腺病毒抗原和兔抗血清的反免疫电泳（CE）相关。对测试程序本身的统计分析表明，测试具有足够的可重复性，可以进一步分析这些参数对腺病毒CE测试的相互作用或联合影响。硫酸盐浓度、EE与CE呈三次多项式关系；硫酸根与EE呈线性关系。抗性与CE有一定的线性关系，但琼脂之间的差异太小，不能显示出明显的相关性。硫酸盐阴离子的固定电荷是主要的，尽管可能不是唯一的，EE的贡献者。通过分光光度计扫描和手测载玻片的EE计算，我们预测了新批琼脂糖在诊断腺病毒CE测试中的有效性，根据推导出的公式，CE评分= 4.9 + 112.1 EE - 397.1 EE2 + 329.1 EE3。腺病毒CE试验的最佳稳定培养基为硫酸盐浓度0.37% +-。0.16%， EE阴极迁移率0.17 +-。0.03.
The sulfate concentration, electroendosmosis (EE), and electrical resistance of 23 commonly used agars and agaroses were measured by standard techniques and related to counterimmunoelectrophoresis (CE) with adenovirus antigens and [rabbit] antisera. Statistical analyses of the test procedures themselves showed that the tests had sufficient reproducibility to warrant further analyses on the interaction or combined effects of these parameters on adenovirus CE tests. Sulfate concentration and EE were related to CE by cubic polynomial equations; sulfate was directly related to EE by a linear equation. Resistance was somewhat linearly related to CE, but the differences among agars were too small to show a clear correlation. The fixed charges of the sulfate anion are major, although probably not the only, contributors to EE. The combined EE calculations from spectrophotometrically-scanned and hand-measured slides were used to predict the usefulness of new lots of agaroses for diagnostic adenovirus CE tests, according to the derived equation, CE score = 4.9 + 112.1 EE - 397.1 EE2 + 329.1 EE3. The best stabilizing media for adenovirus CE tests had sulfate concentrations of 0.37% .+-. 0.16% and EE cathodic mobility ratios of 0.17 .+-. 0.03.