Gastrointestinal Candida colonisation promotes sensitisation against food antigens by affecting the mucosal barrier in mice
Gastrointestinal Candida colonisation promotes sensitisation against food antigens by affecting the mucosal barrier in mice
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DOI：
10.1136/gut.2005.084954
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发表时间：
2006-07-01
期刊：
影响因子：
24.5
通讯作者：
Sonoyama, K.
中科院分区：
文献类型：
--
作者：
Yamaguchi, N.;Sugita, R.;Sonoyama, K.
背景和目标：关于白色念珠菌在胃肠道的定植是否会加重特应性皮炎的病情，仍存在争议。我们假设念珠菌定植促进食物过敏，这是已知的特应性皮炎的致病反应。我们测试了这一点，使用最近建立的小鼠念珠菌定植model.Methods：念珠菌在胃肠道定植建立了胃内接种白色念珠菌喂养的合成饮食的小鼠。为了研究对食物抗原的致敏作用，小鼠每隔一天灌胃给予卵清蛋白，持续9周，每周测量抗卵清蛋白抗体滴度。检查胃肠道的食物抗原的渗透，卵清蛋白的血浆浓度测定胃内给药的ovalbumin.Results：卵清蛋白特异性IgG和IgE滴度较高，在BALB/c小鼠念珠菌定植比正常小鼠。卵清蛋白在BALB/c小鼠胃肠道中的渗透通过定殖而增强。组织学检查显示，定殖促进肥大细胞的浸润和脱颗粒。念珠菌定殖并没有增加卵清蛋白渗透肥大细胞缺陷的W/W-v小鼠，但在同类同窝对照+/+小鼠。重建的肥大细胞在W/W-V小鼠骨髓来源的肥大细胞移植恢复的能力，增加卵清蛋白渗透响应Candida colonisation.Conclusions：这些结果表明，胃肠道念珠菌定植促进对食物抗原的致敏，至少部分是由于肥大细胞介导的小鼠胃肠道粘膜通透性过高。
Backgrounds and aims: Controversy still exists as to whether gastrointestinal colonisation by Candida albicans contributes to aggravation of atopic dermatitis. We hypothesised that Candida colonisation promotes food allergy, which is known to contribute to a pathogenic response in atopic dermatitis. We tested this using a recently established murine Candida colonisation model.Methods: Candida colonisation in the gastrointestinal tract was established by intragastric inoculation with C albicans in mice fed a synthetic diet. To investigate sensitisation against food antigen, mice were intragastrically administered with ovalbumin every other day for nine weeks, and antiovalbumin antibody titres were measured weekly. To examine gastrointestinal permeation of food antigen, plasma concentrations of ovalbumin were measured following intragastric administration of ovalbumin.Results: Ovalbumin specific IgG and IgE titres were higher in BALB/c mice with Candida colonisation than in normal mice. Gastrointestinal permeation of ovalbumin was enhanced by colonisation in BALB/c mice. Histological examination showed that colonisation promoted infiltration and degranulation of mast cells. Candida colonisation did not enhance ovalbumin permeation in mast cell deficient W/W-v mice but did in congenic littermate control +/+ mice. Reconstitution of mast cells in W/W-v mice by transplantation of bone marrow derived mast cells restored the ability to increase ovalbumin permeation in response to Candida colonisation.Conclusions: These results suggest that gastrointestinal Candida colonisation promotes sensitisation against food antigens, at least partly due to mast cell mediated hyperpermeability in the gastrointestinal mucosa of mice.