THE FINE-STRUCTURE OF THE RAT SUBTHALAMIC NUCLEUS - AN ELECTRON-MICROSCOPIC STUDY
THE FINE-STRUCTURE OF THE RAT SUBTHALAMIC NUCLEUS - AN ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1002/cne.902210110
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发表时间：
1983-01-01
影响因子：
2.5
通讯作者：
KITAI, ST
中科院分区：
文献类型：
--
作者：
CHANG, HT;KITA, H;KITAI, ST
对大鼠丘脑底核（STH）的正常超微结构进行了研究。STH由紧密堆积的神经元组成，神经元分布在充满大量血管和稀疏的有髓纤维的神经元中。STH神经元的胞体（直径，D，在10和25 μ m之间）含有丰富的细胞器，但仅具有少量的光滑和粗糙内质网。细胞核内陷较深，核质苍白，异染色质较少。STH神经元通常紧密贴壁，没有任何干预胶质膜。胞体与树突、树突与树突、树突与轴突起始段之间也有类似的排列。虽然经常观察到粘附点，但在这些膜贴壁上均未发现缝隙连接。在神经元中，树突大多数是光滑和薄的（D在0.5和1 μ m之间），偶尔有棘状突起或薄的树突附属物。轴突终末至少有2种类型。1型终末（D高达1 μ m）含有中等大小的圆形囊泡（D约45 nm）并形成不对称突触。2型末端通常较大（D高达5 μ m），并且包含圆形和稍微扁平的囊泡（D高达50 nm）。2型终末除了形成相对对称的突触连接外，还经常与其突触后靶点形成粘附连接。其余的轴突终端包括少量的终端与各种形态特征，并可能有一些切向切片的1型和2型终端。因此，在本研究中未将其归类为单独类型。定量分析表明，1型终末主要与细树突形成突触，而2型终末主要与胞体和大树突形成突触。
The normal ultrastructure of the rat subthalamic nucleus (STH) was studied. The STH consisted to tightly packed neurons distributed within a neuropil filled with large numbers of blood vessels and thinly myelinated fibers. The somata of STH neurons (diameters, D, between 10 and 25 .mu.m) contained abundant organelles but had only a small amount of both smooth and rough endoplasmic reticulum. The nuclei had deeply invaginated nuclear envelopes and pale nucleoplasm with little heterochromatin. STH neurons often were tightly apposed without any intervening glial membranes. Similar appositions were found between somata and dendrites, dendrites and dendrites, and dendrites and initial axon segments. Although puncta adherentia were often observed, no gap junctions were found on any of these membrane appositions. In the neuropil, the dendrites were mostly smooth and thin (D between 0.5 and 1 .mu.m) with an occasional stubby spine or thin dendritic appendage. At least 2 types of axon terminals were identified. Type 1 terminals (D up to 1 .mu.m) contained medium-sized round vesicles (D about 45 nm) and formed asymmetrical synapses. Type 2 terminals were often large (D up to 5 .mu.m) and contained both round and slightly flattened vesicles (D up to 50 nm). The type 2 terminals frequently formed adherens junctions with their postsynaptic targets in addition to forming relatively symmetrical synaptic junctions. The remaining axon terminals included a small number of terminals with various morphological characteristics and possibly some tangentially sectioned type 1 and type 2 terminals. Therefore they have not been classified as individual types in this study. A quantitative analysis indicated that the type 1 terminals formed synapses mainly with thin dendrites whereas the type 2 terminals formed synapses mainly with somata and larger dendrites.