Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system
Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system
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DOI：
10.1126/science.aaf0784
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发表时间：
2016-06-10
期刊：
影响因子：
56.9
通讯作者：
Wagner, Anthony D.
中科院分区：
文献类型：
--
作者：
Brown, Thackery I.;Carr, Valerie A.;Wagner, Anthony D.
少突胶质细胞被认为是中枢神经系统（CNS）中功能同质的群体。我们进行了单细胞RNA测序5072细胞的少突胶质细胞谱系从10个地区的小鼠少年和成年中枢神经系统。鉴定了13个不同的群体，其中12个代表从Pdgfra少突胶质细胞前体细胞（OPC）到不同的成熟少突胶质细胞的连续体。分化的初始阶段是相似的整个青少年中枢神经系统，而成熟的少突胶质细胞的子集富集在特定的区域在成年人的大脑。新形成的少突胶质细胞在成年中枢神经系统中检测到，并对复杂的运动学习作出反应。在沿着血管发现了不同于OPC的第二个Pdgfra'群体。我们的研究揭示了少突胶质细胞分化和成熟的动力学，在转录水平上将它们解偶联，并突出了CNS中少突胶质细胞的异质性。
Oligodendrocytes have been considered as a functionally homogeneous population in the central nervous system (CNS). We performed single-cell RNA sequencing on 5072 cells of the oligodendrocyte lineage from 10 regions of the mouse juvenile and adult CNS. Thirteen distinct populations were identified, 12 of which represent a continuum from Pdgfra' oligodendrocyte precursor cells (OPCs) to distinct mature oligodendrocytes. Initial stages of differentiation were similar across the juvenile CNS, whereas subsets of mature oligodendrocytes were enriched in specific regions in the adult brain. Newly formed oligodendrocytes were detected in the adult CNS and were responsive to complex motor learning. A second Pdgfra' population, distinct from OPCs, was found along vessels. Our study reveals the dynamics of oligodendrocyte differentiation and maturation, uncoupling them at a transcriptional level and highlighting oligodendrocyte heterogeneity in the CNS.