Characterization of the pectin methyl-esterase gene family and its function in controlling pollen tube growth in pear (Pyrus bretschneideri)
Characterization of the pectin methyl-esterase gene family and its function in controlling pollen tube growth in pear (Pyrus bretschneideri)
复制标题
果胶甲酯酶基因家族的特征及其在控制梨花粉管生长中的功能（Pyrus bretschneideri）
DOI：
10.1016/j.ygeno.2020.01.021
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发表时间：
2020
期刊：
影响因子：
4.4
通讯作者：
Shaoling Zhang
中科院分区：
文献类型：
--
作者：
Chao Tang;Xiaoxuan Zhu;Xin Qiao;Hongru Gao;Qionghou Li;Peng Wang;Juyou Wu;Shaoling Zhang
果胶甲酯酶（PMEs）在植物生长中起着重要作用。在本研究中，我们鉴定了梨中的81 PbrPME。全基因组复制和纯化选择驱动了PbrPME基因家族的进化。47个PbrPMEs在梨花粉管中均有表达，表达趋势分析将其分为13个簇。13个簇中的一个呈现相反的表达趋势，对甲基酯化果胶在顶端细胞壁的变化。PbrPMEs定位于细胞质和质膜。PbrPME11、PbrPME44和PbrPME59的表达抑制了梨花粉管的生长，甲基化果胶在花粉管顶端异常沉积。药理学分析证实，降低PbrPME活动抑制花粉管生长。总体而言，我们探索了PbrPME基因家族的进化特征，找到了控制花粉管生长的关键PbrPME基因，加深了对梨育性调控的认识。
Pectin methyl-esterases (PMEs) play crucial roles in plant growth. In this study, we identified 81PbrPMEsin pear. Whole-genome duplication and purifying selection drove the evolution ofPbrPMEgene family. The expression of 47PbrPMEswas detected in pear pollen tube, which were assigned to 13 clusters by an expression tendency analysis. One of the 13 clusters presented opposite expression trends towards the changes of methyl-esterified pectins at the apical cell wall. PbrPMEs were localized in the cytoplasm and plasma membrane. Repression ofPbrPME11,PbrPME44, andPbrPME59resulted in the inhibition of pear pollen tube growth and abnormal deposition of methyl-esterified pectins at pollen tube tip. Pharmacological analysis confirmed that reduced PbrPME activities repressed the pollen tube growth. Overall, we have explored the evolutionary characteristics ofPbrPMEgene family and found the keyPbrPMEgenes that control the growth of pollen tube, which deepened the understanding of pear fertility regulation.