Comparative study of microbiological monitoring of water-miscible metalworking fluids
Comparative study of microbiological monitoring of water-miscible metalworking fluids
复制标题
水溶性金属加工液微生物监测的比较研究
DOI：
10.1016/j.ibiod.2014.11.015
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
A. Rabenstein
中科院分区：
文献类型：
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作者：
T. Koch;F. Passman;A. Rabenstein
微生物活动是造成水溶性金属加工液（MWF）在使用过程中劣化的主要原因之一。虽然保持MWF在无微生物条件下是不切实际的，但及时、准确和易于解释的微生物污染条件可以大大降低MWF生物降解的风险。本文通过实验室和现场样品的检测，比较了不同条件下的微生物污染监测方法的速度和精度。比较了两种培养试验（平板计数和浸片）和两种三磷酸腺苷（ATP）试验方法。此外，基质辅助激光解吸/电离飞行时间质谱（MALDI-ToF-MS）被引入作为一种方法来鉴定从MWF分离的细菌菌株。结果清楚地表明，MWF监测是需要标准化的采样，测量面积，可确定的细胞计数以及物种群落的分析。
Microbial activity is one of the primary causes of water-miscible metalworking fluid (MWF) deterioration in application. Although it is impractical to maintain MWF under microbe-free conditions, timely, accurate and easily interpreted microbial contamination condition can substantially reduce the risk of MWF biodeterioration. This paper compares different condition monitoring methods detecting the microbial contamination of MWF related to their speed and precision in laboratory and field samples. Two culture tests (plate count and dip-slides) and two adenosine triphosphate (ATP) test methods were compared. Additionally the matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-ToF-MS) is introduced as a method for the identification of bacterial strains isolated from MWF. The results clearly indicate that MWF monitoring is in need of standardization concerning sampling, area of measurement, and analysis of determinable cell counts as well as species communities.