Three-dimensional ion mobility TOFMS analysis of electrosprayed biomolecules
Three-dimensional ion mobility TOFMS analysis of electrosprayed biomolecules
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DOI：
10.1021/ac980059c
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发表时间：
1998-06-01
影响因子：
7.4
通讯作者：
Clemmer, DE
中科院分区：
文献类型：
--
作者：
Hoaglund, CS;Valentine, SJ;Clemmer, DE
离子淌度/质谱技术已被开发出来，可以同时记录多个离子的质量分辨离子淌度分布。该方法涉及一种将电喷雾离子源与注入离子漂移管/飞行时间质谱仪耦合的新仪器。离子混合物中的各个成分通过漂移管中的迁移率差异进行分离，然后通过飞行时间仪器中的质荷比进行分散。质谱仪中的飞行时间比漂移管中的停留时间短得多，因此可以同时记录所有离子的质量分辨离子淌度。结果是包含碰撞截面、质荷比和离子丰度信息的三维光谱。描述了仪器和数据采集系统。给出了电喷雾缓激肽和泛素离子分布的组合离子淌度/飞行时间数据的示例。
An ion mobility/mass spectrometry technique has been developed to record mass-resolved ion mobility distributions for multiple ions simultaneously. The approach involves a new instrument that couples an electrospray ion source to an injected-ion drift tube/time-of-flight mass spectrometer. Individual components in a mixture of ions are separated by mobility differences in a drift tube and subsequently dispersed by mass-to-charge ratios in a time-of-flight instrument. Flight times in the mass spectrometer are much shorter than residence times in the drift tube, making it possible to record mass-resolved ion mobilities for all ions simultaneously. The result is a three-dimensional spectrum that contains collision cross section, mass-to-charge, and ion abundance information. The instrument and data acquisition system are described. Examples of combined ion mobility/time-of-flight data are presented for distributions of electrosprayed bradykinin and ubiquitin ions.