The effect of surgical trauma on circulating free DNA levels in cancer patients-implications for studies of circulating tumor DNA
The effect of surgical trauma on circulating free DNA levels in cancer patients-implications for studies of circulating tumor DNA
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DOI：
10.1002/1878-0261.12729
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发表时间：
2020-06-16
影响因子：
6.6
通讯作者：
Andersen, Claus L.
中科院分区：
文献类型：
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作者：
Henriksen, Tenna V.;Reinert, Thomas;Andersen, Claus L.
治疗后循环肿瘤 DNA (ctDNA) 的检测是残留疾病的新兴标志。 ctDNA 仅占癌症患者体内循环的无细胞 DNA (cfDNA) 的一小部分，这使得 ctDNA 检测变得复杂。创伤引起的 cfDNA 会加剧这种情况。为了指导最佳采血时间，我们研究了结直肠癌或膀胱癌患者手术创伤引起的 cfDNA 的持续时间和程度。对 436 名结直肠癌患者和 47 名肌层浸润性膀胱癌患者在术前和术后 6 周内收集的配对血浆样本中的 DNA 水平进行了定量。为了评估创伤引起的 cfDNA 片段是否比普通 cfDNA 片段长，测定了 91 名患者的短片段 (< 1 kb) 和长片段 (> 1 kb) 的浓度。之前报道的 91 名结直肠癌患者和 47 名膀胱癌患者的 ctDNA 数据被用来评估创伤诱导的 DNA 如何影响 ctDNA 检测。结直肠癌患者（平均三倍）和膀胱癌患者（平均八倍）术后 cfDNA 总水平均增加。两个患者队列的 DNA 水平在手术后 4 周内均显着增加（P = 0.0005 和 P
Detection of circulating tumor DNA (ctDNA) post-treatment is an emerging marker of residual disease. ctDNA constitutes only a minor fraction of the cell-free DNA (cfDNA) circulating in cancer patients, complicating ctDNA detection. This is exacerbated by trauma-induced cfDNA. To guide optimal blood sample timing, we investigated the duration and magnitude of surgical trauma-induced cfDNA in patients with colorectal or bladder cancer. DNA levels were quantified in paired plasma samples collected before and up to 6 weeks after surgery from 436 patients with colorectal cancer and 47 patients with muscle-invasive bladder cancer. To assess whether trauma-induced cfDNA fragments are longer than ordinary cfDNA fragments, the concentration of short (< 1 kb) and long (> 1 kb) fragments was determined for 91 patients. Previously reported ctDNA data from 91 patients with colorectal cancer and 47 patients with bladder cancer were used to assess how trauma-induced DNA affects ctDNA detection. The total cfDNA level increased postoperatively-both in patients with colorectal cancer (mean threefold) and bladder cancer (mean eightfold). The DNA levels were significantly increased up to 4 weeks after surgery in both patient cohorts (P = 0.0005 andP