Expression and alteration of insulin-like growth factor II-messenger RNA in hepatoma tissues and peripheral blood of patients with hepatocellular carcinoma
Expression and alteration of insulin-like growth factor II-messenger RNA in hepatoma tissues and peripheral blood of patients with hepatocellular carcinoma
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DOI：
10.3748/wjg.v11.i30.4655
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发表时间：
2005-08-14
影响因子：
4.3
通讯作者：
Meng, Xian-Yong
中科院分区：
文献类型：
--
作者：
Dong, Zhi-Zhen;Yao, Deng-Fu;Meng, Xian-Yong
目的：探讨血清游离胰岛素样生长因子II(IGF-II)水平和外周血中IGF-II mRNA水平在肝细胞癌诊断和肝外转移监测中的临床价值。方法：分别从肝细胞癌患者、无癌肝病患者、非肝肿瘤患者和健康人外周血中提取总RNA。通过随机引物和逆转录酶合成IGF-II的cDNA，经聚合酶链式反应(PCR)扩增，DNA测序证实。采用酶联免疫吸附试验检测不同肝病患者血清中游离IGF-II水平。结果：RT-PCR扩增的IGF-II基因片段与原设计大小为170bp的片段完全一致，并经测序证实。稀释实验表明，该体系的最低灵敏度为2 ng/L。肝细胞癌组织、癌旁组织和非癌组织中IGF-II mRNA的阳性率分别为100%、53.3%和0%。肝细胞癌患者血清游离IGF-II水平明显高于慢性肝炎和肝硬化组。肝细胞癌患者外周血中IGF-II mRNA阳性率为34.2%，其他肝病、肝外肿瘤和正常对照组均未见扩增片段。血清IGF-II的表达与肝细胞癌的分期有关，在有肝外转移的肝癌中阳性率为100%，在AFP阴性的肝癌中阳性率为35.5%。结论：游离IGF-II和IGF-II mRNA的异常表达可作为肝癌诊断、鉴别肝外转移和监测术后复发的有用的肿瘤标志物。(C)2005年WJG Press和Elsevier Inc.保留所有权利。
AIM: To investigate the clinical values of serum free insulin-like growth factor II (IGF-II) levels and IGF-II mRNA in hepatocellular carcinoma (HCC) tissues and peripheral blood for diagnosis of HCC and monitoring of extrahepatic metastasis.METHODS: Total RNAs were extracted from HCC tissues or peripheral blood mononuclear cells from patients with HCC, liver diseases devoid of cancer, non-hepatic tumors, and healthy controls, respectively. IGF-II cDNAs were synthesized through random primers and reverse-transcriptase, amplified by polymerase chain reaction (PCR), and confirmed by DNA sequencing analysis. Serum free IGF-II levels in patients with different liver diseases were analyzed by an enzyme-linked immunosorbent assay.RESULTS: The amplified fragments of IGF-II mRNA by RT-PCR were identical to originally designed ones with a size of 170 bp and confirmed by sequencing analysis. The dilution experiments revealed that the lowest sensitivity of our system was 2 ng/L of total RNA. The positive frequencies of IGF-II mRNA were 100% in HCC tissues, 53.3% in para-cancerous tissues, and 0% in non-cancerous tissues, respectively. The serum free IGF-II levels were significantly higher in HCC than those in chronic hepatitis or liver cirrhosis. The positive frequency of circulating IGF-II mRNA was 34.2% in HCC, no amplified fragment was found in other liver diseases, extrahepatic tumors, and normal controls, respectively. The circulating IGF-II mRNA correlated with the stage of HCC, and its positive rate was 100% in HCC with extrahepatic metastasis and 35.5% in HCC with AFP-negative. No significant correlation was found between tumor sizes and circulating IGF-II mRNA fragment.CONCLUSION: The abnormal expressions of free IGF-II and IGF-II mRNA are useful tumor markers for HCC diagnosis, differentiation of extrahepatic metastasis and monitoring postoperative recurrence. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.