Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine producing bacterium, Morganella psychrotolerans,
Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine producing bacterium, Morganella psychrotolerans,
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过氧乙酸对组胺产生菌、耐冷摩根氏菌的消毒效果及抗菌机制
DOI：
10.1016/j.lwt.2020.109263
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发表时间：
2020
影响因子：
6
通讯作者：
and K. Yamazaki
中科院分区：
文献类型：
--
作者：
Wang;D.;S. Yamaki;Y. Kawai;and K. Yamazaki
在这项研究中，我们旨在研究过氧乙酸(PAA)的消毒作用，以及其对摩根士丹利耐药菌的抑菌作用的潜在机制。暴露量。耐冷剂20ppm PAA作用5min可使其降至检出值以下，表明PAA具有显著的抗菌作用。耐冷性试管。此外，可行性也很重要。耐冷性秋刀鱼(Cololabis Saira)表面经80ppm PAA处理1min和5min后，分别减少了1.40和2.23logCFU/cm2。此外，PAA处理延缓了Fm的生长。4℃下贮藏过程中秋刀鱼的耐冷性。其次，PAA对霉菌的抑菌机理。用扫描电子显微镜和荧光显微镜观察PAA处理对细胞膜和细胞表面的损伤。并对PAA处理后的染色体DNA和蛋白质谱进行分析。根据我们的结果，我们假设PAA处理的杀菌作用主要归因于对细菌细胞膜的破坏。这些结果表明，PAA可能是一种有效的抗支原体消毒剂。耐冷剂在海产品加工和储存中有应用。
In this study, we aimed to investigate the sanitizing effects of peracetic acid (PAA), and the underlying mechanism of its antimicrobial action against Morganellapsychrotolerans. The exposure ofM. psychrotoleransto 20 ppm PAA for 5 min led to its decrease below the detection level, indicating that PAA has a significant antibacterial effect againstM. psychrotoleransinvitro. Moreover, the viable counts ofM. psychrotoleranson saury (Cololabis saira) surface were showed a reduction of 1.40 and 2.23 log CFU/cm2upon treatment with 80 ppm PAA for 1 and 5 min, respectively. Further, PAA treatment delayed the growth ofM. psychrotoleranson saury surface during storage at 4 °C. Next, the antimicrobial mechanism of PAA againstM. psychrotoleranscells was investigated, and the damage to the cell membrane and cell surface upon PAA treatment was observed using scanning electron microscopy (SEM) and epifluorescence microscopy. The chromosomal DNA and the protein profiles after PAA treatment were also analyzed. Form our results, we hypothesized that the bactericidal effect of PAA treatment was mainly attributed to damage the bacterial cell membrane. These results indicate that PAA may be an efficient disinfectant againstM. psychrotoleransand has applications in seafood processing and storage.