DAPI - A DNA-SPECIFIC FLUORESCENT-PROBE
DAPI - A DNA-SPECIFIC FLUORESCENT-PROBE
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DOI：
10.3109/10520299509108199
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发表时间：
1995-09-01
影响因子：
1.6
通讯作者：
KAPUSCINSKI, J
中科院分区：
文献类型：
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作者：
KAPUSCINSKI, J
DAPI（4'，6-二氨基-2-苯基吲哚）是一种DNA特异性探针，通过附着在富含a - t序列的DNA小丛中形成荧光复合物。它也与双链核酸形成非荧光插入复合物。介绍了该染料的物理化学性质及其与核酸的配合物，以及该染料作为生物染色剂的发展历史。综述了DAPI作为DNA特异性探针在细胞计数、染色体染色、DNA可视化和定量等组织化学和生物化学中的应用，讨论了DAPI核酸复合物形成的机制，包括小槽结合、插层和缩合。
DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and its complexes with nucleic acids and history of the development of this dye as a biological stain are described. The application of DAPI as a DNA-specific probe for now cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry is reviewed, The mechanisms of DAPI-nucleic acid complex formation including minor groove binding, intercalation and condensation are discussed.