Three-dimensional microcomputed tomography of renal vasculature in rats
Three-dimensional microcomputed tomography of renal vasculature in rats
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DOI：
10.1161/01.hyp.31.1.440
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发表时间：
1998-01-01
期刊：
影响因子：
8.3
通讯作者：
Romero, JC
中科院分区：
文献类型：
--
作者：
Garcia-Sanz, A;Rodriguez-Barbero, A;Romero, JC
目前观察肾脏微血管的显微镜方法仅显示肾脏总体积的非常有限的一部分。在与钠排泄变化相关的功能状态下，识别皮质和髓质中肾小球后血管的连通性将有助于更好地了解肾血管系统与肾小管功能的耦合。本研究的目的是探讨应用三维微型计算机断层扫描(Micro-CT)显示肾小球前后血管系统连续性的可能性。正常大鼠肾脏在生理压力下原位灌流染色，填充含铬酸铅乳胶微粉，包埋于塑料中。完整肾脏的微型CT扫描是在旋转工作台上进行的，由同步加速器X射线源或传统的X射线光谱管照明。图像通过滤波反投影算法重建，并利用体积绘制技术显示血管系统。重建图像清楚地显示了肾脏的大分布血管和静脉引流，而肾小球前、后血管及其在整个肾脏的血管连接则显示在座位细节上。传出小动脉在皮质区和髓质区表现出管周毛细血管床的特征。皮质血管体积为27%，外髓外纹为18%，外髓内纹为30%，内髓为18%。综上所述，Micro-CT是一种与生理和病理改变相关的评价肾脏血管构筑的有前景的方法。
Current microscopic methods to view renal microvasculature reveal only a very limited portion of the total renal volume. Identification of connectivity for postglomerular vessels in the cortex and the medulla during functional states related to changes in sodium excretion will help better to understand the coupling of renal vasculature to tubular function. The purpose of this study was to investigate the possibility of visualizing the continuity of pre-and postglomerular vasculature using three-dimensional micro-computed tomography (micro-CT). Kidneys from normal rats were perfusion hued in situ at physiological pressure, filled with latex microfil containing lead chromate, and embedded in plastic. The micro-CT scans of the intact kidneys were carried out on a rotating stage illuminated either by a synchrotron x-ray source or a conventional x-ray spectroscopy tube. Images were reconstructed by a filtered backprojection algorithm and volume-rendering techniques were utilized to display the vasculature. The reconstructed images clearly showed the large distribution vessels and the venous drainage of the kidneys, while pre-and postglomerular vessels and their vascular connections throughout the kidney were displayed in seat detail. Efferent arterioles showed the characteristics of their peritubular capillary beds in the cortical and medullary regions. The vascular volume of the cortex was 27%, the outer stripe of the outer medulla 18%, the inner stripe of the outer medulla 30%, and the inner medulla 18%. In conclusion, micro-CT is a promising method to evaluate renal vascular architecture relative to physiological and pathological alterations.