Characterization of damaged skin by impedance spectroscopy: mechanical damage.
Characterization of damaged skin by impedance spectroscopy: mechanical damage.
复制标题
通过阻抗谱表征受损皮肤：机械损伤。
DOI：
10.1007/s11095-013-1052-1
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发表时间：
2013
影响因子：
3.7
通讯作者：
Bunge,AnnetteL
中科院分区：
文献类型：
--
作者：
White,ErickA;Orazem,MarkE;Bunge,AnnetteL
目的电化学阻抗谱是一种简便的方法，已被用来表征皮肤屏障功能，影响药物进入和通过皮肤。本研究的目的是将机械损伤引起的皮肤屏障功能的变化与阻抗谱的变化联系起来。这些观察结果进行了比较，在一个同伴的文件中的化学损伤skin.MethodsElectrical阻抗和非极性化合物的渗透测量之前和之后，人体尸体皮肤被损坏的针puncture.ResultsThe阻抗响应的所有皮肤样品是一致的等效电路模型与电阻器和恒相元件（CPE）并联。针孔损伤的皮肤表现出较低的电阻路径平行的皮肤电阻，而不改变CPE的行为。针刺后确定的阻抗扫描的特征频率增加了一个可以预测的量。4-cyanophenol的通量增加了一个小的，但显着的量，不相关的孔电阻计算的假设下，周围的皮肤的电阻没有changed.ConclusionsSkin阻抗测量是敏感的不可逆的损伤所造成的暴露穿刺用针。
PurposeElectrochemical impedance spectroscopy is a convenient method that has been used to characterize skin barrier function, which affects drug delivery into and through the skin. The objective of this study was to relate changes in skin barrier function arising from mechanical damage to changes in the impedance spectra. These observations are compared in a companion paper to changes in chemically damaged skin.MethodsElectrical impedance and the permeation of a non-polar compound were measured before and after human cadaver skin was damaged by needle puncture.ResultsThe impedance responses of all skin samples were consistent with an equivalent circuit model with a resistor and constant phase element (CPE) in parallel. Pinhole-damaged skin exhibited a lower resistance pathway acting in parallel with the skin resistance without changing the CPE behavior. The characteristic frequency of the impedance scans determined after needle puncture increased by an amount that could be predicted. The flux of 4-cyanophenol increased by a small but significant amount that did not correlate with the hole resistance calculated under the assumption that the resistance of the surrounding skin did not change.ConclusionsSkin impedance measurements are sensitive to irreversible damage caused by exposure to puncture with a needle.