A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice cultivar Cho-Ko-Koku
A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice cultivar Cho-Ko-Koku
复制标题
DOI：
10.1007/s00122-009-1244-6
复制
发表时间：
2010-04-01
影响因子：
5.4
通讯作者：
Akagi, Hiromori
中科院分区：
文献类型：
--
作者：
Tezuka, Kouichi;Miyadate, Hidenori;Akagi, Hiromori
重金属镉（Cd）对人类具有剧毒，可以从受污染的农田进入食物链。了解作物中Cd积累的分子机制将有助于生产安全的无Cd食品。在这里，我们发现了一个单隐性基因，允许水稻更高的镉易位，也确定了该基因的染色体位置。在水培条件下，Cd超积累型品种秋果果的Cd转位比非积累型品种秋田63高3.5倍。从这些品种衍生的F-2群体分析表明，高、低镉易位的分离比为1:3。这表明单隐性基因控制高镉易位表型。QTL分析鉴定出一个QTL， qCdT7，位于7号染色体上。在镉污染田中，F-2种群的镉积累呈连续变化，且有较大的海侵。鉴定出3个Cd积累QTL，其中最有效QTL的峰与qCdT7定位在同一区域。结果表明，qCdT7基因介导的Cd易位在污染土壤Cd积累中起重要作用。
The heavy metal cadmium (Cd) is highly toxic to humans and can enter food chains from contaminated crop fields. Understanding the molecular mechanisms of Cd accumulation in crop species will aid production of safe Cd-free food. Here, we identified a single recessive gene that allowed higher Cd translocation in rice, and also determined the chromosomal location of the gene. The Cd hyperaccumulator rice variety Cho-Ko-Koku showed 3.5-fold greater Cd translocation than the no-accumulating variety Akita 63 under hydroponics. Analysis of an F-2 population derived from these cultivars gave a 1:3 segregation ratio for high:low Cd translocation. This indicates that a single recessive gene controls the high Cd translocation phenotype. A QTL analysis identified a single QTL, qCdT7, located on chromosome 7. On a Cd-contaminated field, Cd accumulation in the F-2 population showed continuous variation with considerable transgression. Three QTLs for Cd accumulation were identified and the peak of the most effective QTL mapped to the same region as qCdT7. Our data indicate that Cd translocation mediated by the gene on qCdT7 plays an important role in Cd accumulation on contaminated soil.