Vertebral trabecular bone microscopic tissue elastic modulus and hardness do not change in ovariectomized rats
Vertebral trabecular bone microscopic tissue elastic modulus and hardness do not change in ovariectomized rats
复制标题
DOI：
10.1002/jor.1100180224
复制
发表时间：
2000-03-01
影响因子：
2.8
通讯作者：
Goldstein, SA
中科院分区：
文献类型：
--
作者：
Guo, XE;Goldstein, SA
切除卵巢的大鼠作为绝经后骨质疏松症的动物模型已被广泛使用。然而，对大鼠椎骨的显微组织特性知之甚少。特别是在骨质疏松症引起的雌激素消耗。本研究应用纳米压痕技术定量测定去卵巢大鼠椎小梁骨组织的显微力学特性。17周大的斯普拉格·道利大鼠接受了卵巢切除术。37周死亡后，用扫描电镜测量腰椎（L4）骨小梁面积的比例，用纳米压痕技术测定弹性模量和硬度。卵巢切除术后骨面积分数明显降低。微观上，弹性模量和硬度变化不明显。结果表明，雌激素依赖性骨质疏松症大鼠表现为骨量减少，而存活骨组织的弹性和硬度保持相对不变。
Ovariectomized rats have been used extensively and have received substantial acceptance as animal models for postmenopausal osteoporosis. However, little is known about the microscopic tissue properties of rat vertebral bone. especially during osteoporosis caused by estrogen depletion. This study applied a new nanoindentation technique to quantify the microscopic mechanical properties of vertebral trabecular bone tissue in ovariectomized rats. Seventeen-week-old Sprague Dawley rats underwent an ovariectomy. After death at 37 weeks, the fraction of the trabecular bone area of the lumbar vertebrae (L4) was measured with scanning electron microscopy and the elastic modulus and hardness were determined with the nanoindentation technique. The bone area fraction was reduced significantly after the ovariectomy. However, the elastic modulus and hardness did not change significantly at the microscopic level. The results indicate that estrogen-dependent osteoporosis in rats manifests in a loss of bone mass whereas the elastic and hardness properties of the surviving bone tissue remain relatively unchanged.