Th17-cells in atopic dermatitis stimulate orthodontic root resorption.
Th17-cells in atopic dermatitis stimulate orthodontic root resorption.
复制标题
特应性皮炎中的 Th17 细胞刺激正畸牙根吸收。
作者：
Yamada K;Yamaguchi M;Asano M;FujitaS;Kobayashi R;Kasai K.
目的探讨异位性皮炎(AD)在正畸牙齿移动过程中对牙根吸收的影响。材料与方法通过对异位性皮炎模型小鼠和野生型小鼠施加过大的正畸力来诱导上颌第一磨牙移动。免疫组织化学方法检测抗酒石酸酸性磷酸酶(TRAP)、IL-17、IL-6和RANKL蛋白在牙周膜中的表达水平。结果AD患者外周血中TRAP、IL-17、IL-6、RANKL和RANKL的免疫反应活性明显增强。IL-17/CD4免疫荧光双标分析检测免疫反应。AD患者IL-17、IL-6和RANKL的分泌以及IL-6和RANKL的mRNA水平均高于健康人。结论Th17细胞可能与AD小鼠牙根吸收的恶化有关，并可能解释了AD患者在过量应用OF时更易发生牙根吸收的原因。
ObjectiveThe aim of this study was to investigate how atopic dermatitis (AD) contributes to root resorption during orthodontic tooth movement.Materials and MethodsAtopic dermatitis model mice and wild‐type mice were subjected to an excessive orthodontic force (OF) to induce movement of the upper first molars. The expression levels of the tartrate‐resistant acid phosphatase (TRAP), IL‐17, IL‐6, and RANKL proteins were determined in the periodontal ligament (PDL) by an immunohistochemical analysis. Furthermore, the effects of the compression force on co‐cultures of CD4+cells from AD patients or healthy individuals and human PDL cells were investigated with regard to the levels of secretion and mRNA expression of IL‐17, IL‐6, RANKL, and osteoprotegerin.ResultsThe immunoreactivities for TRAP, IL‐17, IL‐6, and RANKL in the AD group were found to be significantly increased. The double immunofluorescence analysis for IL‐17/CD4 detected immunoreaction. The secretion of IL‐17, IL‐6, and RANKL, and the mRNA levels of IL‐6 and RANKL in the AD patients were increased compared with those in healthy individuals.ConclusionTh17 cells may therefore be associated with the deterioration of root resorption of AD mice, and may explain why AD patients are more susceptible to root resorption than healthy individuals when an excessive OF is applied.