Migrating neural crest cells in the trunk of the avian embryo are multipotent.
Migrating neural crest cells in the trunk of the avian embryo are multipotent.
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发表时间：
1991-08
期刊：
影响因子：
4.6
通讯作者：
Scott E. Fraser;M. Bronner‐Fraser
中科院分区：
文献类型：
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作者：
Scott E. Fraser;M. Bronner‐Fraser
干神经嵴细胞广泛迁移，并产生不同的细胞类型，包括感觉和自主神经系统的细胞。在此之前，我们证明了许多迁移前干神经嵴细胞产生的后代在多个神经嵴衍生物具有不同的表型。结果是一致的想法，神经嵴细胞是多能的，然后从神经管移民，并成为限制在表型后离开神经管无论是在其迁移过程中或在其网站的本地化。在这里，我们测试的发展潜力的迁移躯干神经嵴细胞显微注射的重要染料，赖氨酸罗丹明葡聚糖（LRD），到个别细胞，因为它们通过体节迁移。注射后两天，LRD标记的克隆含有2至67个细胞，这些细胞单侧分布在所有胚胎中。大多数克隆局限于一个单一的节段，虽然有几个贡献交感神经节超过两个节段。大多数克隆产生了多个神经嵴衍生物的细胞。个别迁移的神经嵴细胞引起的感觉和交感神经元（神经递质阳性），以及与许旺细胞的形态特征的细胞，和其他非神经元细胞（神经递质阴性）。即使是那些只产生一种神经嵴衍生物的克隆，也常常同时含有神经元特异性阳性和神经元特异性阴性细胞。我们的数据表明，迁移的躯干神经嵴细胞可以是多能的，引起细胞在多个神经嵴衍生物，并有助于神经元和非神经元元素在一个给定的衍生物。（250字处删节）
Trunk neural crest cells migrate extensively and give rise to diverse cell types, including cells of the sensory and autonomic nervous systems. Previously, we demonstrated that many premigratory trunk neural crest cells give rise to descendants with distinct phenotypes in multiple neural crest derivatives. The results are consistent with the idea that neural crest cells are multipotent prior to their emigration from the neural tube and become restricted in phenotype after leaving the neural tube either during their migration or at their sites of localization. Here, we test the developmental potential of migrating trunk neural crest cells by microinjecting a vital dye, lysinated rhodamine dextran (LRD), into individual cells as they migrate through the somite. By two days after injection, the LRD-labelled clones contained from 2 to 67 cells, which were distributed unilaterally in all embryos. Most clones were confined to a single segment, though a few contributed to sympathetic ganglia over two segments. A majority of the clones gave rise to cells in multiple neural crest derivatives. Individual migrating neural crest cells gave rise to both sensory and sympathetic neurons (neurofilament-positive), as well as cells with the morphological characteristics of Schwann cells, and other non-neuronal cells (both neurofilament-negative). Even those clones contributing to only one neural crest derivative often contained both neurofilament-positive and neurofilament-negative cells. Our data demonstrate that migrating trunk neural crest cells can be multipotent, giving rise to cells in multiple neural crest derivatives, and contributing to both neuronal and non-neuronal elements within a given derivative.(ABSTRACT TRUNCATED AT 250 WORDS)