Differential staining of dysplastic aberrant crypt foci in the colon facilitates prediction of carcinogenic potentials of chemicals in rats
Differential staining of dysplastic aberrant crypt foci in the colon facilitates prediction of carcinogenic potentials of chemicals in rats
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DOI：
10.1016/j.canlet.2004.10.026
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发表时间：
2005-03-18
期刊：
影响因子：
9.7
通讯作者：
Nakagama, H
中科院分区：
文献类型：
--
作者：
Ochiai, M;Watanabe, M;Nakagama, H
我们开发了一种新的和简单的方法，以确定不典型增生的异常隐窝病灶（ACF）诱导的大鼠结肠致癌物更有效和选择性，而不进行费力的组织学检查，这通常需要足够的时间才能得到最终诊断。在常规的0.2%亚甲基蓝染色后，增加一个简单的70%甲醇脱色过程，可以区分异常ACF。为了检验这种新方法的有效性，我们称之为差异染色，我们分析了三种杂环胺诱导的结肠病变，包括2-氨基-1甲基-6-苯基咪唑[4，5-B]吡啶，发现检测到的异型增生ACF的数量比ACF的总数更准确地反映了它们的致癌潜力。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
We developed a novel and simple method to identify dysplastic aberrant crypt foci (ACF) induced in rats by colon carcinogens more efficiently and selectively without conducting laborious histological examination, which usually requires enough time to get final diagnosis. By adding a simple decolorization process with 70% methanol after conventional 0.2% methylene blue staining, dysplastic ACF could be differentially contrasted. To examine the validity of this novel method, which we refer to as differential staining, we analyzed colonic lesions induced by three heterocyclic amines, including 2-amino-lmethyl-6-phenylimidazo[4,5-b]pyridine, and found that the number of dysplastic ACF detected more precisely reflected their carcinogenic potential than the total numbers of ACF. (c) 2005 Elsevier Ireland Ltd. All rights reserved.