Direct and selective tagging of cysteine residues in peptides and proteins with 4-nitropyridyl lanthanide complexes
Direct and selective tagging of cysteine residues in peptides and proteins with 4-nitropyridyl lanthanide complexes
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DOI：
10.1039/c3cc45875j
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发表时间：
2013-01-01
影响因子：
4.9
通讯作者：
Parker, David
中科院分区：
文献类型：
--
作者：
Gempf, Kathryn L.;Butler, Stephen J.;Parker, David
报道了一种水中半胱氨酸选择性标记方法，该方法基于简单吡啶和镧系元素络合物中吡啶基硝基取代基的快速置换。缀合反应在标签和肽之间创建了一个短连接，使肽更接近 Ln(3+) 离子，并且与现有方法相比灵活性降低。
A cysteine-selective tagging method in water is reported, based on rapid displacement of a pyridyl nitro-substituent in simple pyridines and lanthanide complexes. The conjugation reaction creates a short link between the tag and peptide, holding the peptide closer to the Ln(3+) ion and with reduced flexibility compared to existing methods.