Poly(decyl methacrylate)-based fluorescent PEBBLE swarm nanosensors for measuring dissolved oxygen in biosamples
Poly(decyl methacrylate)-based fluorescent PEBBLE swarm nanosensors for measuring dissolved oxygen in biosamples
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DOI：
10.1039/b403086a
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发表时间：
2004-01-01
期刊：
影响因子：
4.2
通讯作者：
Kopelman, R
中科院分区：
文献类型：
--
作者：
Cao, YF;Koo, YEL;Kopelman, R
研制了150-250 nm的聚甲基丙烯酸癸酯（PDMA）荧光比率型溶解氧纳米传感器。铂八乙基卟啉酮（PtOEPK），氧敏感染料，和八乙基卟啉（OEP），氧不敏感的染料，已被纳入到PDMA纳米粒子，使传感器的比例。基于相应的Stern-Volmer图，这些纳米传感器在从0到42.5 ppm的溶解分子氧的整个范围内（脱氧到纯氧鼓泡水）表现出几乎完全的线性。整体猝灭响应高达97.5%，是迄今为止所有溶解氧光学传感器中最好的。这些PEBBLE纳米传感器还显示出非常好的可逆性和稳定性，以浸出和光漂白，以及非常短的响应时间，没有蛋白质的干扰。在人血浆中，它们表现出强大的氧传感能力，几乎不受光散射和自发荧光的影响。潜在的应用包括细胞内氧成像和生物和其他异质环境中的微分辨压力分布。
150-250 nm Poly( decyl methacrylate) ( PDMA) fluorescent ratiometric nanosensors for dissolved oxygen have been developed. Platinum octaethylporphine ketone (PtOEPK), the oxygen-sensitive dye, and octaethylporphyrin (OEP), the oxygen-insensitive dye, have been incorporated into PDMA nanoparticles to make the sensors ratiometric. Based on the corresponding Stern-Volmer plot, these nanosensors exhibit almost complete linearity over the whole range of dissolved molecular oxygen from 0 to 42.5 ppm (deoxygenated to pure oxygen-bubbled water). The overall quenching response is up to 97.5%, the best so far for all dissolved oxygen optical sensors. These PEBBLE nanosensors also show very good reversibility and stability to leaching and photobleaching, as well as very short response times and no perturbation by proteins. In human plasma they demonstrate a robust oxygen sensing capability, little affected by light scattering and autofluorescence. Potential applications include intracellular oxygen imaging and microresolved pressure profiles in biological and other heterogenous environments.