Simultaneous determination of catecholamines, uric acid and ascorbic acid at physiological levels using poly(N-methylpyrrole)/Pd-nanoclusters sensor
Simultaneous determination of catecholamines, uric acid and ascorbic acid at physiological levels using poly(N-methylpyrrole)/Pd-nanoclusters sensor
复制标题
DOI：
10.1016/j.ab.2010.01.001
复制
发表时间：
2010-05-01
影响因子：
2.9
通讯作者：
Galal, Ahmed
中科院分区：
文献类型：
--
作者：
Atta, Nada F.;El-Kady, Maher F.;Galal, Ahmed
在聚N-甲基吡咯（PMPy）膜涂铂（Pt）电极上电沉积钯纳米团簇（Pd-nano），构建了一种有趣的电化学传感器。采用循环伏安法、电化学阻抗谱（EIS）和扫描电镜对修饰电极的性能进行了表征。结果表明，该修饰电极的电活性强烈依赖于PMPy膜和Pd纳米的电合成条件。此外，该修饰电极对多巴胺（DA）、抗坏血酸（AA）和尿酸（UA）混合物的氧化具有较强的电催化活性，过电位明显降低.在常规电极（Pt、金[Au]和玻璃碳）上同时分析这种混合物通常很困难。然而，三个良好分辨的氧化峰AA，DA和UA与大的峰分离允许该修饰电极单独或同时分析AA，DA和UA通过使用差分脉冲伏安法（DPV）具有良好的稳定性，灵敏度和选择性。该传感器也是同时分析AA、UA和肾上腺素（E）、去甲肾上腺素（NE）或L-DOPA的理想选择。此外，该传感器对乙酰氨基酚（ACOP）和其他有机化合物显示出很强的电催化活性。AA，DA和UA的校准曲线分别在0.05至1 mM，0.1至10 μ M和0.5至20 μ M的范围内获得。AA、DA和UA的检测限（信噪比[S/N] = 3）分别为7 μ M、12 nM和27 nM。将该修饰电极应用于注射液、人血清和人尿样中AA、DA和UA的测定，结果令人满意。该修饰电极的可靠性和稳定性为该技术应用于临床检测AA、DA和UA的常规分析提供了良好的可能性。(C)2010年爱思唯尔公司All rights reserved.
An interesting electrochemical sensor has been constructed by the electrodeposition of palladium nanoclusters (Pd-nano) on poly(N-methylpyrrole) (PMPy) film-coated platinum (Pt) electrode. Cyclic voltammetry, electrochemical impedance spectroscopy (EIS), and scanning electron microscopy were used to characterize the properties of the modified electrode. It was demonstrated that the electroactivity of the modified electrode depends strongly on the electrosynthesis conditions of the PMPy film and Pd-nano. Moreover, the modified electrode exhibits strong electrocatalytic activity toward the oxidation of a mixture of dopamine (DA), ascorbic acid (AA), and uric acid (UA) with obvious reduction of overpotentials. The simultaneous analysis of this mixture at conventional (Pt, gold [Au], and glassy carbon) electrodes usually struggles. However, three well-resolved oxidation peaks for AA, DA, and UA with large peak separations allow this modified electrode to individually or simultaneously analyze AA, DA, and UA by using differential pulse voltammetry (DPV) with good stability, sensitivity, and selectivity. This sensor is also ideal for the simultaneous analysis of AA, UA and either of epinephrine (E), norepinephrine (NE) or L-DOPA. Additionally, the sensor shows strong electrocatalytic activity towards acetaminophen (ACOP) and other organic compounds. The calibration curves for AA, DA, and UA were obtained in the ranges of 0.05 to 1 mM, 0.1 to 10 mu M, and 0.5 to 20 mu M, respectively. The detection limits (signal/noise [S/N] = 3) were 7 mu M, 12 nM, and 27 nM for AA, DA, and UA, respectively. The practical application of the modified electrode was demonstrated by measuring the concentrations of AA, DA, and UA in injection sample, human serum, and human urine samples, respectively, with satisfactory results. The reliability and stability of the modified electrode gave a good possibility for applying the technique to routine analysis of AA, DA, and UA in clinical tests. (C) 2010 Elsevier Inc. All rights reserved.