Simultaneously constructing nanotopographical and chemical cues in 3D-printed polylactic acid scaffolds to promote bone regeneration
Simultaneously constructing nanotopographical and chemical cues in 3D-printed polylactic acid scaffolds to promote bone regeneration
复制标题
在 3D 打印的聚乳酸支架中同时构建纳米拓扑结构和化学线索以促进骨再生
DOI：
10.1016/j.msec.2020.111457
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Li Ji-Hua
中科院分区：
文献类型：
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作者：
Wang Peng;Yin Hua-Mo;Li Xiang;Liu Wei;Chu Yu-Xian;Wang Yu;Wang Yan;Xu Jia-Zhuang;Li Zhong-Ming;Li Ji-Hua
局部结构和生物活性表面涂层可有效改善骨再生的生物学功能。然而，将这些益处同时引入到三维（3D）多孔支架中提出了令人生畏的挑战。在这项研究中，我们提出了一种简单而有效的方法，用化学修饰的纳米形貌图案装饰3D打印的聚乳酸（PLA）支架。通过在醇/碱溶液中蚀刻PLA的无定形相以暴露致密的层来产生纳米形貌。随后，共形装饰的聚多巴胺（PDA）实现通过自组装的儿茶酚胺，而不改变表面的纳米形貌。包括活细胞和死细胞染色、细胞形态学、细胞生长和碱性磷酸酶在内的体外细胞实验表明，纳米形貌和PDA涂层的组合导致成骨细胞在3D打印支架中的粘附、扩散和增殖的有利增强。使用大鼠股骨临界尺寸缺损模型在体内评价整合模式对骨再生的贡献。Micro-CT评价和组织学分析表明，与裸支架和仅用纳米形貌修饰的支架相比，用集成图案修饰的支架促进成骨。我们提出的方法为提高骨组织再生三维聚合物支架的生物活性提供了一种很有前途的方法。
Topographical structures and bioactive surface coatings are effective in improving the biological function for bone regeneration. However, the simultaneous introduction of these benefits into three-dimensional (3D) porous scaffolds poses a daunting challenge. In this study, we proposed a simple yet effective approach to decorate 3D-printed polylactic acid (PLA) scaffolds with chemically modified nanotopographical patterns. The nanotopography was produced by etching the amorphous phase of PLA in an alcohol/alkali solution to expose dense lamellae. Subsequently, conformal decoration of polydopamine (PDA) was realized via self-assembly of catecholamines without changing the surface nanotopography. In vitro cell experiments including live and dead staining, cell morphology, cell growth, and alkaline phosphatase showed that the combination of nanotopography and PDA-coating led to a favorable enhancement of osteoblasts adhesion, spread and proliferation in 3D-printed scaffolds. The contribution of integrated patterns to bone regeneration was evaluated using a rat femur critical-sized defect model in vivo. Micro-CT evaluation and histological analysis demonstrated that the scaffold decorated with integrated pattens promoted osteogenesis more than the bare scaffolds and the scaffolds decorated with only nanotopography. Our proposed approach offers a promising method for improving bioactivity of 3D polymer scaffolds for bone tissue regeneration.