Rapid Single Cell Printing by Piezoelectric Inkjet Printer
Rapid Single Cell Printing by Piezoelectric Inkjet Printer
复制标题
压电喷墨打印机快速单细胞打印
DOI：
10.1007/978-4-431-55139-3_3
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
K. Morishima
中科院分区：
文献类型：
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作者：
R. The;S. Yamaguchi;A. Ueno;Y. Akiyama;K. Morishima
组织工程正在迅速发展，以协助治疗器官丧失或组织损伤。另一方面，相对较长的药物开发过程也可能需要体外制造器官进行药物测试，这为开发过程打开了更快的大门。一些方法和技术已经被引入作为组织工程的有价值的工具，而喷墨打印从21世纪初开始作为“无支架”组织工程工具脱颖而出，尽管无支架的特性仍然存在争议。此外，该技术还被用于将单个细胞封装在液滴中，从而为在开放空间中分离单个细胞提供了可能，并在个体水平上更好地控制细胞打印的时空。结合喷墨打印机的高频液滴喷射能力，高通量单细胞阵列和更精细的组织制造的高速制造在未来也可能成为可能。本章介绍了在任意图案上沉积细胞的几种方法，并探讨了在任意图案上沉积细胞和单细胞喷墨打印技术实现中的挑战。本章还讨论了相对较大的颗粒和细胞打印的一些调整和限制，它们与商业喷墨打印机中的油墨颗粒的尺寸规模不同。
Tissue engineering is rapidly developing to assist in the treatment of organ loss or tissue damage. On the other hand, the relatively long process of drug development may also need in vitro fabricated organ for drug testing, which opens up faster door to development process. Several methods and technologies have been introduced as valuable tools for tissue engineering, and inkjet printing stands out as the “scaffold-less” tissue engineering tools starting from the early 2000s, although the scaffold-less feature is still debatable. Furthermore, this technology was also used to encapsulate single cells in the fluid droplet, thus opening up the possibility for isolation of single cells in the open space and better spatiotemporal control of cell printing at individual level. Combined with the high-frequency droplet ejection capability of inkjet printer, high-throughput single cells array and high-speed fabrication of finer detail tissue fabrication may also be possible in the future. This chapter introduced several methods used to deposit cells on arbitrary pattern and explored on the challenge in the implementation of inkjet printing technology to deposit cells and single cells on arbitrary pattern. This chapter also argued several adjustments and limitations for relatively large particles and cell printing, which are in a different size scale compared to ink particles in the commercial inkjet printer.