Olfactory rod cells: a rare cell type in the larval zebrafish olfactory epithelium with an actin-rich apical projection
Olfactory rod cells: a rare cell type in the larval zebrafish olfactory epithelium with an actin-rich apical projection
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嗅杆细胞：斑马鱼幼虫嗅上皮中的一种罕见细胞类型，具有富含肌动蛋白的顶端突起
DOI：
10.1101/2020.11.04.367979
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Cheung K
中科院分区：
文献类型：
--
作者：
Cheung K
我们报告存在一种罕见的细胞类型，嗅杆细胞，在发育中的斑马鱼嗅觉上皮。这些细胞每个都有一个单一的富含肌动蛋白的杆状突起，从上皮表面延伸5-10 μm。使用普遍存在的Lifeact-RFP标签进行实时成像表明，嗅杆可以振荡。嗅杆在嗅坑打开后的几个小时内出现，在幼虫阶段增加数量和大小，并且可以在没有嗅纤毛的情况下发育。嗅杆状细胞在形态上不同于已知的嗅觉感觉神经元，但由神经元启动子驱动表达报告。嗅棒细胞亚群表达由ox10启动子驱动的Lifeact-mRFPruby转基因。该基因的嵌合表达表明，嗅杆细胞具有位于嗅上皮顶端的圆形细胞体，没有可检测到的轴突。我们在讨论中对这些细胞的可能功能进行了推测。
We report the presence of a rare cell type, the olfactory rod cell, in the developing zebrafish olfactory epithelium. These cells each bear a single actin-rich rod-like apical projection extending 5–10 μm from the epithelial surface. Live imaging with a ubiquitous Lifeact-RFP label indicates that the olfactory rods can oscillate. Olfactory rods arise within a few hours of the olfactory pit opening, increase in numbers and size during larval stages, and can develop in the absence of olfactory cilia. Olfactory rod cells differ in morphology from the known classes of olfactory sensory neuron, but express reporters driven by neuronal promoters. A sub-population of olfactory rod cells expresses a Lifeact-mRFPruby transgene driven by thesox10promoter. Mosaic expression of this transgene reveals that olfactory rod cells have rounded cell bodies located apically in the olfactory epithelium and have no detectable axon. We offer speculation on the possible function of these cells in the Discussion.